Evidence map›Paper›PMID 37938391›Full record

ArticleWorld journal of microbiology & biotechnology2023

Multidrug Efflux System-mediated resistance in Staphylococcus aureus under a One Health approach.

Mariana de Barros, Ilderlane da Silva Lopes, Ana Júlia Moreira, Raquel Dos Santos Oliveira Almeida, Mateus Matiuzzi da Costa, Rinaldo Aparecido Mota, Luis Augusto Nero, Maria Aparecida Scatamburlo Moreira

Abstract read
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In one paragraph

Article in World journal of microbiology & biotechnology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.2field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Mariana de BarrosDepartment of Veterinary, Universidade Federal de Viçosa, Viçosa, MG, Brazil.ORCID http://orcid.org/0000-0001-6956-0864
Ilderlane da Silva LopesDepartment of Veterinary, Universidade Federal de Viçosa, Viçosa, MG, Brazil.ORCID http://orcid.org/0000-0002-5893-5795
Ana Júlia MoreiraDepartment of Veterinary, Universidade Federal de Viçosa, Viçosa, MG, Brazil.ORCID http://orcid.org/0000-0001-8090-0655
Raquel Dos Santos Oliveira AlmeidaDepartment of Veterinary, Universidade Federal de Viçosa, Viçosa, MG, Brazil.
Mateus Matiuzzi da CostaUniversidade Federal do Vale do São Francisco, Petrolina, PE, Brazil.ORCID http://orcid.org/0000-0002-9884-2112
Rinaldo Aparecido MotaUniversidade Federal Rural de Pernambuco, Recife, PE, Brazil.ORCID http://orcid.org/0000-0002-2844-5509
Luis Augusto NeroDepartment of Veterinary, Universidade Federal de Viçosa, Viçosa, MG, Brazil.ORCID http://orcid.org/0000-0002-4954-5824
Maria Aparecida Scatamburlo MoreiraDepartment of Veterinary, Universidade Federal de Viçosa, Viçosa, MG, Brazil. masm@ufv.br.ORCID http://orcid.org/0000-0002-6902-2502
Universidade Federal de Viçosa · BRUniversidade Federal do Vale do São Francisco · BRUniversidade Federal Rural de Pernambuco · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The aim of the study was to track the spread of antimicrobial resistance among the different sectors of One Health through the detection of Multidrug-Efflux-System in multidrug-resistant Staphylococcus aureus isolates. Multidrug-resistant (MDR) and methicillin-resistant (MRSA) S. aureus isolates were selected: 25 of human, one of animal and eight of food origin. The efflux system genes norA, norB, norC, LmrS, tet38 and msrA were screened by PCR. The activity of the efflux systems was determined by the minimum inhibitory concentration (MIC) of tetracycline and ciprofloxacin in the presence and absence of CCCP and in the quantification of ethidium bromide efflux. Furthermore, biofilm formation was determined in the presence and absence of the CCCP. The molecular epidemiology of the isolates was traced with the aid of PFGE. The gene norC was the most prevalent, detected in all isolates and msrA was the least prevalent, detected in only two isolates from humans. There was no difference in the MICs of tetracycline and ciprofloxacin in the presence of CCCP, but 55.9% of isolates showed ethidium bromide efflux. The presence of CCCP decreased the biofilm formation. Regarding the molecular epidemiology, in three clusters was a mixture of the isolates from different origins. Therefore, S. aureus MDR with active multidrug efflux systems are circulating between One Health domains and it is necessary to consider strategies to decrease this circulation in order to prevent the dissemination of resistance mediated by MES.

Indexed as

Methicillin-Resistant Staphylococcus aureusOne HealthStaphylococcal InfectionsAnimalsAnti-Bacterial AgentsCarbonyl Cyanide m-Chlorophenyl HydrazoneCiprofloxacinEthidiumHumansStaphylococcus aureusTetracyclineAnti-Bacterial AgentsCarbonyl Cyanide m-Chlorophenyl HydrazoneCiprofloxacinEthidiumTetracyclineAntibiotic resistanceGenetic characterizationGram-positiveOne Medicine

Identifiers

PMID37938391
OpenAlexW4388496397

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.