ArticleISME communications2021
Microvolume DNA extraction methods for microscale amplicon and metagenomic studies.
Article in ISME communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
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- Quantitative metagenomics using a portable protocol.Applied and environmental microbiology · 2026Article
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- Defining the ecological strategies of phytoplankton associated bacteria.Nature communications · 2025Article
- Chemotaxis, growth, and inter-species interactions shape early bacterial community assembly.The ISME journal · 2025Article
- From microscale to microbial insights: validating high-throughput microvolume extraction (HiMEx) methods for marine microbial ecology.ISME communications · 2025Article
- Plant miRNAs influence soil bacterial growth and amino acid uptake, restructuring community composition.ISME communications · 2025Article
- Widespread production of plant growth-promoting hormones among marine bacteria and their impacts on the growth of a marine diatom.Microbiome · 2024Article
- Microscale sampling of the coral gastrovascular cavity reveals a gut-like microbial community.Animal microbiome · 2024Article
- The coral microbiome in sickness, in health and in a changing world.Nature reviews. Microbiology · 2024Review
- Assessment of nucleic acid extraction protocols for antibiotic resistance genes (ARGs) quantification in aircraft wastewater.Human genomics · 2024Article
- SecMet-FISH: labeling, visualization, and enumeration of secondary metabolite producing microorganisms.FEMS microbiology ecology · 2024Article
- Solid Phase Oligo-DNA Extraction from Complex Medium Using an Aminated Graphene/Nitrocellulose Membrane Hybrid.Biomolecules · 2024Article
- A targeted approach to enrich host-associated bacteria for metagenomic sequencing.FEMS microbes · 2024Article
- Strong chemotaxis by marine bacteria towards polysaccharides is enhanced by the abundant organosulfur compound DMSP.Nature communications · 2023Article
- Compact and automated eDNA sampler for in situ monitoring of marine environments.Scientific reports · 2023Article
- Chemotaxis may assist marine heterotrophic bacterial diazotrophs to find microzones suitable for NThe ISME journal · 2022Article
- Article
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Authors and funding
7 authors.
Funding
Abstract
Investigating the composition and metabolic capacity of aquatic microbial assemblages usually requires the filtration of multi-litre samples, which are up to 1 million-fold larger than the microenvironments within which microbes are predicted to be spatially organised. To determine if community profiles can be reliably generated from microlitre volumes, we sampled seawater at a coastal and an oceanic site, filtered and homogenised them, and extracted DNA from bulk samples (2 L) and microvolumes (100, 10 and 1 μL) using two new approaches. These microvolume DNA extraction methods involve either physical or chemical lysis (through pH/thermal shock and lytic enzymes/surfactants, respectively), directly followed by the capture of DNA on magnetic beads. Downstream analysis of extracted DNA using both amplicon sequencing and metagenomics, revealed strong correlation with standard large volume approaches, demonstrating the fidelity of taxonomic and functional profiles of microbial communities in as little as 1 μL of seawater. This volume is six orders of magnitude smaller than most standard operating procedures for marine metagenomics, which will allow precise sampling of the heterogenous landscape that microbes inhabit.
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Registered trials
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