ArticleThe Journal of cell biology1987
Chromosomes move poleward in anaphase along stationary microtubules that coordinately disassemble from their kinetochore ends.
Article in The Journal of cell biology, 1987. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 118 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
118 citing papers in PubMed, 315 citations in OpenAlex.
- Structure and function of the outer kinetochore.Nature reviews. Molecular cell biology · 2026Review
- Mechanical coordination between anaphase A and B drives asymmetric chromosome segregation.bioRxiv : the preprint server for biology · 2025Article
- Optimal strategies for correcting merotelic chromosome attachments in anaphase.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- CKAP5 stabilizes CENP-E at kinetochores by regulating microtubule-chromosome attachments.EMBO reports · 2024Article
- A general model for the motion of multivalent cargo interacting with substrates.Journal of the Royal Society, Interface · 2023Article
- Microtubule Sliding within the Bridging Fiber Pushes Kinetochore Fibers Apart to Segregate Chromosomes.Developmental cell · 2017Article
- Congressing kinetochores progressively load Ska complexes to prevent force-dependent detachment.The Journal of cell biology · 2017Article
- Review
- A Brief History of Research on Mitotic Mechanisms.Biology · 2016Review
- Miguel Mota (1922-2016)-the kinetochore engine(er).Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2016Article
- Mitosis, microtubule dynamics and the evolution of kinesins.Experimental cell research · 2015Review
- From equator to pole: splitting chromosomes in mitosis and meiosis.Genes & development · 2015Review
- Medicinal Plants: A Potential Source of Compounds for Targeting Cell Division.Drug target insights · 2015Article
- Direct kinetochore-spindle pole connections are not required for chromosome segregation.The Journal of cell biology · 2014Article
- Prime movers: the mechanochemistry of mitotic kinesins.Nature reviews. Molecular cell biology · 2014Review
- Regulation of chromosome speeds in mitosis.Cellular and molecular bioengineering · 2013Article
- Searching for electrical properties, phenomena and mechanisms in the construction and function of chromosomes.Computational and structural biotechnology journal · 2013Article
- Biophysics of mitosis.Quarterly reviews of biophysics · 2012Review
- The molecular basis of anaphase A in animal cells.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2011Article
- CLASP1, astrin and Kif2b form a molecular switch that regulates kinetochore-microtubule dynamics to promote mitotic progression and fidelity.The EMBO journal · 2010Article
58 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
During the movement of chromosomes in anaphase, microtubules that extend between the kinetochores and the poles shorten. We sought to determine where subunits are lost from these microtubules during their shortening. Prophase or prometaphase cells on coverslips were injected with fluoresceinated tubulin and allowed to progress through mitosis. Immediately after the onset of anaphase, a bar-shaped beam of laser light was used to mark a domain on the kinetochore fibers by photobleaching a band, approximately 1.0 micron wide, across the spindle. In different cells, spindles were photobleached at varying distances from the chromosomes. Cells were allowed to continue in anaphase until the chromosomes had further separated. They were then lysed, fixed, and prepared for double-label immunofluorescence with an antibody to fluorescein that does not bind appreciably to bleached fluorescein, and with an antibody to tubulin. Photobleached domains of microtubules appeared as bands of reduced fluorescence in the anti-fluorescein image. However, the anti-tubulin labeling revealed that microtubules were present and continuous through the photobleached domains. In all cases, the chromosomes approached and invaded the bleached domain while the bleached domain itself remained stationary with respect to the near pole. These results demonstrate that the chromosomes move along stationary kinetochore microtubules and that depolymerization of these microtubules during anaphase takes place at the kinetochore. In contrast to the generally accepted older view that chromosomes are passive objects pulled by "traction fibers," we suggest that the kinetochore is an active participant in generating the motive force that propels the chromosome to the pole.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.