Evidence map›Paper›PMID 37937256›Full record

ArticleJournal of biological methods2023

Benchmarking assembly free nanopore read mappers to classify complex millipede gut microbiota via Oxford Nanopore Sequencing Technology.

Orlando J Geli-Cruz, Carlos J Santos-Flores, Matias J Cafaro, Alex Ropelewski, Alex R Van Dam

Abstract read
In one paragraph

Article in Journal of biological methods, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Orlando J Geli-CruzUniversidad de Puerto Rico, Recinto Universitario de Mayagüez, Call Box 9000 Mayagüez, PR 00681-9000.
Carlos J Santos-FloresUniversidad de Puerto Rico, Recinto Universitario de Mayagüez, Call Box 9000 Mayagüez, PR 00681-9000.
Matias J CafaroUniversidad de Puerto Rico, Recinto Universitario de Mayagüez, Call Box 9000 Mayagüez, PR 00681-9000.
Alex RopelewskiPittsburgh Supercomputing Center, 300 S. Craig Street, Pittsburgh, PA 15213.
Alex R Van DamUniversidad de Puerto Rico, Recinto Universitario de Mayagüez, Call Box 9000 Mayagüez, PR 00681-9000.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Millipedes are key players in recycling leaf litter into soil in tropical ecosystems. To elucidate their gut microbiota, we collected millipedes from different municipalities of Puerto Rico. Here we aim to benchmark which method is best for metagenomic skimming of this highly complex millipede microbiome. We sequenced the gut DNA with Oxford Nanopore Technologies' (ONT) MinION sequencer, then analyzed the data using

Indexed as

DNA extractionGut microbiotaMetagenomicsMyriapodaNanopore sequencing

Identifiers

PMID37937256
PMCPMC10627078

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-SA
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.