Evidence map›Paper›PMID 37936199›Full record

ArticleStem cell research & therapy2023

Comparison of osteoclast differentiation protocols from human induced pluripotent stem cells of different tissue origins.

Alexander Blümke, Erica Ijeoma, Jessica Simon, Rachel Wellington, Medania Purwaningrum, Sergei Doulatov, Elizabeth Leber, Marta Scatena, Cecilia M Giachelli

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell research & therapy, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
1.5field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 10 citations in OpenAlex.

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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 3 countries.

Alexander BlümkeDepartment of Bioengineering, Department of Medicine, University of Washington, Foege Hall University of Washington, 3720 15th, Ave NE, Box 355061, Seattle, WA, 98195, USA.
Erica IjeomaDepartment of Bioengineering, Department of Medicine, University of Washington, Foege Hall University of Washington, 3720 15th, Ave NE, Box 355061, Seattle, WA, 98195, USA.
Jessica SimonDepartment of Bioengineering, Department of Medicine, University of Washington, Foege Hall University of Washington, 3720 15th, Ave NE, Box 355061, Seattle, WA, 98195, USA.
Rachel WellingtonDivision of Hematology, Department of Medicine, University of Washington, Seattle, WA, USA.
Medania PurwaningrumDepartment of Bioengineering, Department of Medicine, University of Washington, Foege Hall University of Washington, 3720 15th, Ave NE, Box 355061, Seattle, WA, 98195, USA.
Sergei DoulatovDivision of Hematology, Department of Medicine, University of Washington, Seattle, WA, USA.
Elizabeth LeberDepartment of Bioengineering, Department of Medicine, University of Washington, Foege Hall University of Washington, 3720 15th, Ave NE, Box 355061, Seattle, WA, 98195, USA.
Marta ScatenaDepartment of Bioengineering, Department of Medicine, University of Washington, Foege Hall University of Washington, 3720 15th, Ave NE, Box 355061, Seattle, WA, 98195, USA.
Cecilia M GiachelliDepartment of Bioengineering, Department of Medicine, University of Washington, Foege Hall University of Washington, 3720 15th, Ave NE, Box 355061, Seattle, WA, 98195, USA. ceci@uw.edu.ORCID 0000-0002-6387-5993
University of Washington · USUniversitas Gadjah Mada · ID

Funding

Mechanisms of vascular and valvular calcificationR35HL139602 · NHLBI · UNIVERSITY OF WASHINGTON · PI GIACHELLI, CECILIA M · 2018 to 2023
$5.6M
Molecular and Cellular Regulation of Vascular CalcificationR35HL171342 · NHLBI · UNIVERSITY OF WASHINGTON · PI Cecilia M Giachelli · 2024 to 2026
$3.1M
NHLBI NIH HHS R35 HL139602NHLBI NIH HHS R35 HL171342
6 · The paper itself

Abstract

backgroundEver since their discovery, induced pluripotent stem cells (iPSCs) have been extensively differentiated into a large variety of cell types. However, a limited amount of work has been dedicated to differentiating iPSCs into osteoclasts. While several differentiation protocols have been published, it remains unclear which protocols or differentiation methods are preferable regarding the differentiation of osteoclasts.

methodsIn this study, we compared the osteoclastogenesis capacity of a peripheral blood mononuclear cell (PBMC)-derived iPSC line to a fibroblast-derived iPSC line in conjunction with either embryoid body-based or monolayer-based differentiation strategies. Both cell lines and differentiation protocols were investigated regarding their ability to generate osteoclasts and their inherent robustness and ease of use. The ability of both cell lines to remain undifferentiated while propagating using a feeder-free system was assessed using alkaline phosphatase staining. This was followed by evaluating mesodermal differentiation and the characterization of hematopoietic progenitor cells using flow cytometry. Finally, osteoclast yield and functionality based on resorptive activity, Cathepsin K and tartrate-resistant acid phosphatase (TRAP) expression were assessed. The results were validated using qRT-PCR throughout the differentiation stages.

resultsEmbryoid body-based differentiation yielded CD45

conclusionsThe outcome of this study demonstrates the successful differentiation of osteoclasts from iPSCs in conjunction with the embryoid-based differentiation method, while the monolayer-based method did not yield osteoclasts. No differences were observed regarding osteoclast differentiation between the PBMC and fibroblast-derived iPSC lines.

Indexed as

Induced Pluripotent Stem CellsCathepsin KCell DifferentiationHumansLeukocytes, MononuclearOsteoclastsCathepsin KHematopoietic differentiationHuman induced pluripotent stem cellsMesodermal differentiationMineral resorptionOsteoclastogenesisOsteoclasts

Identifiers

PMID37936199
PMCPMC10631132
OpenAlexW4388464931

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.