Evidence map›Paper›PMID 37936064›Full record

ArticleBMC plant biology2023

Comprehensive analysis of the UDP-glucuronate decarboxylase (UXS) gene family in tobacco and functional characterization of NtUXS16 in Golgi apparatus in Arabidopsis.

Zhimin Li, Runping Chen, Yufang Wen, Hanxiang Liu, Yangyang Chen, Xiaoyu Wu, Youxin Yang, Xinru Wu, Yong Zhou, Jianping Liu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC plant biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.4field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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3 · Its place in the literature

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0 citing papers in PubMed, 1 citations in OpenAlex.

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4 · The record

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5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Zhimin Li *Jiangxi Engineering Laboratory for the Development and Utilization of Agricultural Microbial Resources, College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang, China.
Runping Chen *College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang, China.
Yufang WenCollege of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang, China.
Hanxiang LiuCollege of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang, China.
Yangyang ChenCollege of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang, China.
Xiaoyu WuJiangxi Engineering Laboratory for the Development and Utilization of Agricultural Microbial Resources, College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang, China.
Youxin YangJiangxi Key Laboratory for Postharvest Technology and Nondestructive Testing of Fruits & Vegetables, Collaborative Innovation Center of Post-Harvest Key Technology and Quality Safety of Fruits and Vegetables, College of Agronomy, Jiangxi Agricultural University, Nanchang, China.
Xinru WuTobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, China.
Yong ZhouJiangxi Engineering Laboratory for the Development and Utilization of Agricultural Microbial Resources, College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang, China. yongzhou@jxau.edu.cn.
Jianping LiuJiangxi Engineering Laboratory for the Development and Utilization of Agricultural Microbial Resources, College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang, China. JianpingLiu@jxau.edu.cn.
Jiangxi Agricultural University · CNTobacco Research Institute · CN

Funding

Education Department of Jiangxi Province GJJ190204National Natural Science Foundation of China 32060047National Natural Science Foundation of China 32160219
6 · The paper itself

Abstract

backgroundUDP-glucuronate decarboxylase (also named UXS) converts UDP-glucuronic acid (UDP-GlcA) to UDP-xylose (UDP-Xyl) by decarboxylation of the C6-carboxylic acid of glucuronic acid. UDP-Xyl is an important sugar donor that is required for the synthesis of plant cell wall polysaccharides.

resultsIn this study, we first carried out the genome-wide identification of NtUXS genes in tobacco. A total of 17 NtUXS genes were identified, which could be divided into two groups (Group I and II), and the Group II UXSs can be further divided into two subgroups (Group IIa and IIb). Furthermore, the protein structures, intrachromosomal distributions and gene structures were thoroughly analyzed. To experimentally verify the subcellular localization of NtUXS16 protein, we transformed tobacco BY-2 cells with NtUXS16 fused to the monomeric red fluorescence protein (mRFP) at the C terminus under the control of the cauliflower mosaic virus (CaMV) 35S promoter. The fluorescent signals of NtUXS16-mRFP were localized to the medial-Golgi apparatus. Contrary to previous predictions, protease digestion analysis revealed that NtUXS16 is not a type II membrane protein. Overexpression of NtUXS16 in Arabidopsis seedling in darkness led to a significant increase in hypocotyl length and a reduction in root length compared with the wild type. In summary, these results suggest Golgi apparatus localized-NtUXS16 plays an important role in hypocotyl and root growth in the dark.

conclusionOur findings facilitate our understanding of the novel functions of NtUXS16 and provide insights for further exploration of the biological roles of NtUXS genes in tobacco.

Indexed as

ArabidopsisArabidopsis ProteinsCarboxy-LyasesGlucuronatesGlucuronic AcidGolgi ApparatusNicotianaUridine Diphosphate XyloseArabidopsis ProteinsCarboxy-LyasesGlucuronatesGlucuronic AcidUridine Diphosphate XyloseGolgi apparatusHypocotyl lengthRoot lengthTobaccoUDP-glucuronate decarboxylase

Identifiers

PMID37936064
PMCPMC10631120
OpenAlexW4388497809

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LicenceCC BY
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.