ArticleCommunications biology2023
High-level production of nervonic acid in the oleaginous yeast Yarrowia lipolytica by systematic metabolic engineering.
Article in Communications biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 33 citations in OpenAlex.
- Toward Complex Polyketide Biosynthesis inJournal of microbiology and biotechnology · 2026Review
- Biosynthesis of Sciadonic Acid Derived from Gymnosperms with Anti-Colitis Activity.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Metabolic Pathways and Molecular Regulatory Mechanisms of Nervonic Acid Biosynthesis inInternational journal of molecular sciences · 2026Review
- Carbon Dioxide Fixation Cascade for the Production of Single-Cell Protein byJournal of microbiology and biotechnology · 2026Article
- EngineeringSynthetic and systems biotechnology · 2026Article
- A Synergistic Genetic Engineering Strategy Induced Nervonic Acid inInternational journal of molecular sciences · 2026Article
- The Development and Characterization of a Nervonic-Acid-Rich Structured Lipid.Molecules (Basel, Switzerland) · 2026Article
- Impact of Ethanol Stress onFoods (Basel, Switzerland) · 2025Article
- Effects of Adding Hydroxytyrosol to the Diet of Pigs in the Nursery Phase on Growth Performance, Biochemical Markers, and Fatty Acid Profile.Animals : an open access journal from MDPI · 2025Article
- Systematic metabolic engineering ofSynthetic and systems biotechnology · 2025Article
- Nervonic acid in infant nutrition: a forward-looking approach to enhancing neurodevelopmental outcomes.Frontiers in nutrition · 2025Review
- Recent advances and future directions on GLA-producing organisms.Frontiers in bioengineering and biotechnology · 2025Review
- Nervonic Acid Synthesis Substrates as Essential Components in Profiled Lipid Supplementation for More Effective Central Nervous System Regeneration.International journal of molecular sciences · 2024Review
- Key enzymes involved in the utilization of fatty acids byFrontiers in microbiology · 2023Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nervonic acid benefits the treatment of neurological diseases and the health of brain. In this study, we employed the oleaginous yeast Yarrowia lipolytica to overproduce nervonic acid oil by systematic metabolic engineering. First, the production of nervonic acid was dramatically improved by iterative expression of the genes ecoding β-ketoacyl-CoA synthase CgKCS, fatty acid elongase gELOVL6 and desaturase MaOLE2. Second, the biosynthesis of both nervonic acid and lipids were further enhanced by expression of glycerol-3-phosphate acyltransferases and diacylglycerol acyltransferases from Malania oleifera in endoplasmic reticulum (ER). Third, overexpression of a newly identified ER structure regulator gene YlINO2 led to a 39.3% increase in lipid production. Fourth, disruption of the AMP-activated S/T protein kinase gene SNF1 increased the ratio of nervonic acid to lignoceric acid by 61.6%. Next, pilot-scale fermentation using the strain YLNA9 exhibited a lipid titer of 96.7 g/L and a nervonic acid titer of 17.3 g/L (17.9% of total fatty acids), the highest reported titer to date. Finally, a proof-of-concept purification and separation of nervonic acid were performed and the purity of it reached 98.7%. This study suggested that oleaginous yeasts are attractive hosts for the cost-efficient production of nervonic acid and possibly other very long-chain fatty acids (VLCFAs).
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.