ArticleNature communications2023
Single-cell analysis identifies genes facilitating rhizobium infection in Lotus japonicus.
Article in Nature communications, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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Who cites it
29 citing papers in PubMed, 45 citations in OpenAlex.
- Single-Cell RNA-Sequencing in Nodulated Roots of Medicago truncatula.Methods in molecular biology (Clifton, N.J.) · 2027Article
- Nissolia brasiliensis as a nonnodulating model legume.Plant physiology · 2026Article
- A recently evolved Nodulin recruited by NIN for root hair rhizobial infection in Medicago truncatula.The New phytologist · 2026Article
- Cyclophilin A-mediated cis/trans isomerization modulates RIN4 to control intracellular rhizobial infection in legumes.The New phytologist · 2026Article
- Benchmarking plant single cell RNA-sequencing sample processing strategies.The EMBO journal · 2026Article
- Nitrogen-Controlled Host Gatekeeping: Regulatory Chokepoints Across Four Windows for Diazotroph Access.International journal of molecular sciences · 2026Article
- Advances in single-cell and spatial omics for studying symbiotic nitrogen fixation: comparative cellular and evolutionary perspectives.Genome biology · 2026Review
- RHD6LA regulates root hair responses to both symbionts and commensals.Nature communications · 2026Article
- Root cell wall remodeling during symbiotic microbial colonization.Frontiers in plant science · 2026Review
- Why "Where" Matters as Much as "How Much": Single-Cell and Spatial Transcriptomics in Plants.International journal of molecular sciences · 2025Review
- Article
- The Lotus japonicus alpha-expansin EXPA1 is recruited during intracellular and intercellular rhizobial colonization.The Plant journal : for cell and molecular biology · 2025Article
- Single-Cell Omics in Legumes: Research Trends and Applications.Plants (Basel, Switzerland) · 2025Review
- Coordination of cortex modifications in time, space, and under stress.The New phytologist · 2025Review
- Single-nucleus RNA-sequencing reveals the cellular programs driving nematode-induced giant cell formation in tomato.Horticulture research · 2025Article
- Integrated single-cell transcriptomics and spatial metabolomics unveil cellular differentiation and ginsenosides biosynthesis inHorticulture research · 2025Article
- A mobile DELLA controls Medicago truncatula root cortex patterning to host arbuscular mycorrhizal fungi.Nature plants · 2025Article
- Next-generation translational genomics for developing future crops.Functional & integrative genomics · 2025Review
- To immunity and beyond: the central role of jasmonate signalling in beneficial root-microbe-environment interactions.The New phytologist · 2025Review
- Ethylene biosynthesis in legumes: gene identification and expression during early symbiotic stages.Journal of experimental botany · 2025Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Legume-rhizobium signaling during establishment of symbiotic nitrogen fixation restricts rhizobium colonization to specific cells. A limited number of root hair cells allow infection threads to form, and only a fraction of the epidermal infection threads progress to cortical layers to establish functional nodules. Here we use single-cell analysis to define the epidermal and cortical cell populations that respond to and facilitate rhizobium infection. We then identify high-confidence nodulation gene candidates based on their specific expression in these populations, pinpointing genes stably associated with infection across genotypes and time points. We show that one of these, which we name SYMRKL1, encodes a protein with an ectodomain predicted to be nearly identical to that of SYMRK and is required for normal infection thread formation. Our work disentangles cellular processes and transcriptional modules that were previously confounded due to lack of cellular resolution, providing a more detailed understanding of symbiotic interactions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.