Fucose-binding lectins: purification, characterization and potential biomedical applications.

The property of lectins to specifically recognize and bind carbohydrates makes them an excellent candidate in biomedical research. Among them are fucose-binding lectins possessing the capacity to bind fucose are taxonomically, evolutionarily and ecologically significant class of lectins that are identified in a wide range of taxa. Purification of fucose-binding lectins dates back to 1967 when L-fucose binding protein from Lotus tetragonolobus was isolated using a dye that contained three α-L-fucopyranosyl residues. Beginning with that, several FBLs were purified from various animals as well as plant sources that were structurally and functionally characterised. This review focuses on fucose-binding lectins, their occurrence and purification with special emphasis on various strategies adopted to purify them followed by molecular and functional characterization. The exclusive ability to recognize and bind to fucose-containing glycans endows these lectins with the potential to act as anti-cancer agents, diagnostic markers and mitogens for immune cells. Though they have been in research focus for more than half a century with their occurrence reported in various taxa, they still need to be explored for their prospective functions to develop them as a biological tool in biomedical research.