Evidence map›Paper›PMID 37934297›Full record

ArticleArchives of microbiology2023

Transcriptomic analysis approach towards an improved tolerance of Escherichia coli to gallic acid stress.

Lina Liu, Shijie Tang, Tingting Liu, Zihao Zhang, Xuefeng Wang, Muhammad Bilal, Shuai Liu, Hongzhen Luo, Yuping Zhao, Xuguo Duan

Abstract read
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In one paragraph

Article in Archives of microbiology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 46% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Lina LiuSchool of Life Science and Food Engineering, Huaiyin Institute of Technology, Huaian, 223003, China. linaliu@hyit.edu.cn.
Shijie TangSchool of Life Science and Food Engineering, Huaiyin Institute of Technology, Huaian, 223003, China.
Tingting LiuSchool of Life Science and Food Engineering, Huaiyin Institute of Technology, Huaian, 223003, China.
Zihao ZhangSchool of Life Science and Food Engineering, Huaiyin Institute of Technology, Huaian, 223003, China.
Xuefeng WangSchool of Life Science and Food Engineering, Huaiyin Institute of Technology, Huaian, 223003, China.
Muhammad BilalSchool of Life Science and Food Engineering, Huaiyin Institute of Technology, Huaian, 223003, China.
Shuai LiuSchool of Life Science and Food Engineering, Huaiyin Institute of Technology, Huaian, 223003, China.
Hongzhen LuoSchool of Life Science and Food Engineering, Huaiyin Institute of Technology, Huaian, 223003, China.
Yuping ZhaoSchool of Life Science and Food Engineering, Huaiyin Institute of Technology, Huaian, 223003, China.
Xuguo DuanCollege of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing, 210037, Jiangsu, China.
Huaiyin Institute of Technology · CNNanjing Forestry University · CN

Funding

Basic science in colleges and universities of Jiangsu Province 23KJB180001National Natural Science Foundation of China 21808075Natural Science Foundation of Jiangsu Province BK20170459
6 · The paper itself

Abstract

As a natural green additive, gallic acid has been widely used in food production. However, it can inhibit the physiological metabolism of Escherichia coli, which severely limits the ability and efficiency of gallic acid production. To explore the adaptation mechanism of E. coli under gallic acid stress and further explore the target of genetic modification, the effects of gallic acid stress on the fermentation characteristics of E. coli W3110 ATCC (82057) were investigated by cell biomass and cell morphometry. Moreover, transcriptome analysis was used to analyze the gene transcription level of E. coli W3110 ATCC (82057) to explore effects of gallic acid stress on important essential physiological processes. The results showed that under high concentration of gallic acid, the biomass of E. coli W3110 ATCC (82057) decreased significantly and the cells showed irregular morphology. Transcriptome analysis showed that E. coli W3110 ATCC (82057) improved its adaptive capacity through three strategies. First, genes of bamD, ompC, and ompF encoding outer membrane protein BamD, OmpC, and OmpC were decreased 5-, 31.1- and 8.1-fold, respectively, under gallic acid stress compared to the control, leading to the reduction of gallic acid absorption. Moreover, genes (mdtA, mdtB, mdtC, mdtD, mdtE, and mdtF) related to MdtABC multidrug efflux system and multidrug efflux pump MdtEF were up-regulated by1.0-53.0 folds, respectively, and genes (aaeA, aaeB, and aaeX) related to AaeAB efflux system were up-regulated by 8.0-13.3 folds, respectively, which contributed to the excretion of gallic acid. In addition, genes of acid fitness island also were up-regulated by different degrees under the stress of an acidic environment to maintain the stability of the intracellular environment. In conclusion, E. coli W3110 ATCC (82057) would enhance its tolerance to gallic acid by reducing absorption, increasing excretion, and maintaining intracellular environment stability. This study provides research ideas for the construction of engineered strains with high gallic acid yield.

Indexed as

Escherichia coliTranscriptomeBiological TransportGallic AcidGene Expression ProfilingGallic AcidAdaptive mechanismEscherichia coliFermentation characteristicsGallic acidStress

Identifiers

PMID37934297
OpenAlexW4388462848

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.