ArticlePlant biotechnology journal2024
Fine-tuning rice heading date through multiplex editing of the regulatory regions of key genes by CRISPR-Cas9.
Article in Plant biotechnology journal, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
33 citing papers in PubMed.
- Tuning Rice Gene Expression via In Situ Promoter Truncations Using a Prime Editing Library.Plant biotechnology journal · 2026Article
- Grain-maturing temperature induces seed epi-memory via DNA methylation for subsequent development in rice (Oryza sativa L.).Plant physiology · 2026Article
- MeNet: A mixed-effect deep neural network for multi-environment genomic prediction of agronomic traits.Plant communications · 2026Article
- Multiplex gene editing drives revolution in crop breeding: overlaid editing of multiple genes and customization of complex traits.Advanced biotechnology · 2026Review
- Accelerated breeding for early-maturing and aromatic glutinous restorer lines with CRISPR/Cas9-mediated targeted editing for hybrid rice.BMC plant biology · 2026Article
- CRISPR/Cas9-mediated uORF engineering enhances tanshinone biosynthesis inHorticulture research · 2026Article
- Engineering non-coding DNA Elements in Rice: an Elegant Approach To fine-tune Agronomical Advantageous Traits.Rice (New York, N.Y.) · 2025Review
- Allelic value in gene regulation-implications for gene editing.Annals of botany · 2025Article
- JMJ720 regulates flowering time in rice via H3K9 demethylation of Hd1.Plant biotechnology journal · 2025Article
- Genomic signatures of local adaptation to precipitation and solar radiation in kiwifruit.Plant diversity · 2025Article
- A 65-kb deletion survey identifies a distalProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Programmable genome engineering and gene modifications for plant biodesign.Plant communications · 2025Review
- In silico prediction of variant effects: promises and limitations for precision plant breeding.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Review
- Through the lens of bioenergy crops: advances, bottlenecks, and promises of plant engineering.The Plant journal : for cell and molecular biology · 2025Review
- CRISPR-Cas applications in agriculture and plant research.Nature reviews. Molecular cell biology · 2025Review
- Plant engineering: advances, bottlenecks, and promise.The Plant journal : for cell and molecular biology · 2025Article
- Convenient, high-efficiency multiplex genome editing in autotetraploid alfalfa using endogenousaBIOTECH · 2025Article
- Article
- A Series of Novel Alleles ofPlants (Basel, Switzerland) · 2025Article
- Combining AI and new genomic techniques to 'fine-tune' plants: challenges in risk assessment.Frontiers in plant science · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
18 authors.
Funding
Abstract
Heading date (or flowering time) is a key agronomic trait that affects seasonal and regional adaption of rice cultivars. An unoptimized heading date can either not achieve a high yield or has a high risk of encountering abiotic stresses. There is a strong demand on the mild to moderate adjusting the heading date in breeding practice. Genome editing is a promising method which allows more precise and faster changing the heading date of rice. However, direct knock out of major genes involved in regulating heading date will not always achieve a new germplasm with expected heading date. It is still challenging to quantitatively adjust the heading date of elite cultivars with best adaption for broader region. In this study, we used a CRISPR-Cas9 based genome editing strategy called high-efficiency multiplex promoter-targeting (HMP) to generate novel alleles at cis-regulatory regions of three major heading date genes: Hd1, Ghd7 and DTH8. We achieved a series of germplasm with quantitative variations of heading date by editing promoter regions and adjusting the expression levels of these genes. We performed field trials to screen for the best adapted lines for different regions. We successfully expanded an elite cultivar Ningjing8 (NJ8) to a higher latitude region by selecting a line with a mild early heading phenotype that escaped from cold stress and achieved high yield potential. Our study demonstrates that HMP is a powerful tool for quantitatively regulating rice heading date and expanding elite cultivars to broader regions.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.