ArticleBMC genomics2023
Safety assessment of Enterococcus lactis strains complemented with comparative genomics analysis reveals probiotic and safety characteristics of the entire species.
Article in BMC genomics, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 22 citations in OpenAlex.
- Update of the list of qualified presumption of safety (QPS) recommended microbiological agents intentionally added to food or feed as notified to EFSA 24: Suitability of taxonomic units notified to EFSA until March 2026.EFSA journal. European Food Safety Authority · 2026Article
- Comparative genomics reveals population structure and functional differentiation in Limosilactobacillus fermentum.Scientific reports · 2026Article
- Probiotic Apilactobacillus Kunkeei EABW06 from Beeswax Microbiota: A Candidate for Functional Dairy Foods.Probiotics and antimicrobial proteins · 2026Article
- Functional and Genomic insights into probiotic Lactic Acid Bacteria isolated from the Gastrointestinal microbiota of domestic rabbits (Oryctolagus cuniculus).BMC microbiology · 2026Article
- Intestinal Microbiota and Probiotic Characteristics of Two Indigenous Chicken Breeds (Hotan Black Chicken and Baicheng You Chicken) from the Tarim Basin.Animals : an open access journal from MDPI · 2026Article
- Genomic Characterization, Safety Assessment, and Probiotic Potential of a Novel Human-DerivedMicroorganisms · 2026Article
- Genomic safety, bacteriocinogenic potential, and probiotic-associated traits ofFrontiers in microbiology · 2026Article
- Oral Contraceptives Induce Time- and Intestinal Segment-Dependent Shifts in the Gut Microbiota.Nutrients · 2025Article
- Dissecting the High Esterase/Lipase Activity and Probiotic Traits inFoods (Basel, Switzerland) · 2025Article
- Genomic Characterization and Safety Evaluation ofAntibiotics (Basel, Switzerland) · 2025Article
- Article
- Update of the list of qualified presumption of safety (QPS) recommended microbiological agents intentionally added to food or feed as notified to EFSA 21: Suitability of taxonomic units notified to EFSA until September 2024.EFSA journal. European Food Safety Authority · 2025Article
- CRISPR-Cas systems in enterococci.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Review
- Effects of Mink-OriginAnimals : an open access journal from MDPI · 2024Article
- Unraveling the antimicrobial potential ofFrontiers in microbiology · 2024Article
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Authors and funding
4 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe gut microbiota is considered a rich source for potential novel probiotics. Enterococcus genus is a normal component of a healthy gut microbiota, suggesting its vital role. Nosocomial infections caused mainly by E. facalis and E. faecium have been attributed to the plasticity of the Enterococcus genomes. In this study, we assessed the probiotic and safety characteristics of two E. lactis strains isolated from the human gut microbiota using in-vitro and in silico approaches. Additionally, the safety of the E. lactis species was evaluated using comparative genomics analysis.
resultsThe two E. lactis strains 10NA and 50NA showed resistance to bile salts and acid tolerance with antibacterial activity against Escherichia coli, Salmonella typhi, and Clostridioides difficile. For safety assays, the two strains did not display any type of hemolysis on blood agar, and the survival of Caco-2 cells was not significantly different (P-value > 0.05) compared to the control using cell free supernatants at 100% (v/v), 50% (v/v), 10% (v/v), and 5% (v/v) concentrations. Regarding antibiotic susceptibility, both strains were sensitive to vancomycin, tetracycline, and chloramphenicol. Comprehensive whole-genome analysis revealed no concerning associations between virulence or antibiotic resistance genes and any of the identified mobile genetic elements. Comparative genome analysis with closely related E. faecium species genomes revealed the distinctive genomic safety of the E. lactis species.
conclusionsOur two E. lactis strains showed promising probiotic properties in-vitro. Their genomes were devoid of any transferable antibiotic resistance genes. In silico comparative analysis confirmed the safety of the E. lactis species. These results suggest that E. lactis species could be a potential source for safer Enterococcus probiotic supplements.
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