Evidence map›Paper›PMID 37932697›Full record

ArticleBMC genomics2023

Differential expressions of FASN, SCD, and FABP4 genes in the ribeye muscle of omega-3 oil-supplemented Tattykeel Australian White lambs.

John Roger Otto, Shedrach Benjamin Pewan, Richard Crawford Edmunds, Felista Waithira Mwangi, Robert Tumwesigye Kinobe, Oyelola Abdulwasiu Adegboye, Aduli Enoch Othniel Malau-Aduli

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC genomics, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.1field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 6 citations in OpenAlex.

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  4. Effect ofAnimals : an open access journal from MDPI · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 2 countries.

John Roger OttoSchool of Environmental and Life Sciences, College of Engineering, Science and Environment, The University of Newcastle, Callaghan, NSW, 2308, Australia. ajulu04@gmail.com.
Shedrach Benjamin PewanSchool of Environmental and Life Sciences, College of Engineering, Science and Environment, The University of Newcastle, Callaghan, NSW, 2308, Australia.
Richard Crawford EdmundsAustralian Institute of Marine Science, Townsville, QLD, 4810, Australia.
Felista Waithira MwangiSchool of Environmental and Life Sciences, College of Engineering, Science and Environment, The University of Newcastle, Callaghan, NSW, 2308, Australia.
Robert Tumwesigye KinobeCollege of Public Health, Medical and Veterinary Sciences, James Cook University, Townsville, QLD, 4811, Australia.
Oyelola Abdulwasiu AdegboyeMenzies School of Health Research, Charles Darwin University, Casuarina, NT, 0811, Australia.
Aduli Enoch Othniel Malau-AduliSchool of Environmental and Life Sciences, College of Engineering, Science and Environment, The University of Newcastle, Callaghan, NSW, 2308, Australia.
University of Newcastle Australia · AUAustralian Institute of Marine Science · AUCharles Darwin University · AUJames Cook University · AU

Funding

Science Industry Endowment Fund Ross Metcalf STEM Business Fellowship co-funded by Tattykeel Australian White Pty Ltd 00044The Innovation Connections Research Grant from the Australian Commonwealth Government's Department of Industry ICG001084
6 · The paper itself

Abstract

backgroundThe concept of the functional nutritional value of health-beneficial omega-3 long-chain polyunsaturated fatty acids (n-3 LC-PUFA) is becoming a phenomenon among red meat consumers globally. This study examined the expressions of three lipogenic genes (fatty acid binding protein 4, FABP4, fatty acid synthase, FASN; and stearoyl-CoA desaturase, SCD) in the ribeye (Longissimus thoracis et lumborum) muscle of Tattykeel Australian White (TAW) lambs fed fortified omega-3 diets and correlations with fatty acids. To answer the research question, "are there differences in the expression of lipogenic genes between control, MSM whole grain and omega-3 supplemented lambs?", we tested the hypothesis that fortification of lamb diets with omega-3 will lead to a down-regulation of lipogenic genes. Seventy-five six-month old TAW lambs were randomly allocated to the (1) omega-3 oil-fortified grain pellets, (2) unfortified grain pellets (control) or (3) unfortified MSM whole grain pellets diet supplements to generate three treatments of 25 lambs each. The feeding trial lasted 47 days.

resultsFrom the Kruskal-Wallis test, the results showed a striking disparity in lipogenic gene expression between the three dietary treatments in which the FABP4 gene was significantly up-regulated by 3-folds in the muscles of lambs fed MSM Milling (MSM) whole grain diet compared to the omega-3 and control diets. A negative correlation was observed between FASN gene expression and intramuscular fat (IMF), eicosapentaenoic acid (EPA), total polyunsaturated fatty acids (PUFA), omega-6 polyunsaturated fatty acids (n-6 PUFA) and monounsaturated fatty acids (MUFA). The FABP4 gene expression was positively correlated (P < 0.05) with EPA and docosahexaenoic acid (DHA).

conclusionTaken together, this study's results suggest that FABP4 and FASN genes perform an important role in the biosynthesis of fatty acids in the ribeye muscle of TAW lambs, and supplementary diet composition is an important factor influencing their expressions.

Indexed as

Fatty Acids, Omega-3Stearoyl-CoA DesaturaseAnimalsAustraliaDietEicosapentaenoic AcidFatty AcidsFatty Acids, UnsaturatedMusclesSheepSheep, DomesticTranscription FactorsEicosapentaenoic AcidFatty AcidsFatty Acids, Omega-3Fatty Acids, UnsaturatedStearoyl-CoA DesaturaseTranscription FactorsFatty acid binding protein 4Fatty acid synthaseOmega-3 fortified dietsRibeye muscleStearoyl-CoA desaturaseTattykeel Australian WhiteUnsaturated fatty acids

Identifiers

PMID37932697
PMCPMC10626737
OpenAlexW4388413974

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.