ArticleScientific reports2023
Optimizing alpha-amylase from Bacillus amyloliquefaciens on bread waste for effective industrial wastewater treatment and textile desizing through response surface methodology.
Article in Scientific reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 19 papers.
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19 citing papers in PubMed.
- Harnessing shrimp shell waste: enhanced chitinase production through optimization techniques for ecofriendly solutions using bacteria.Biotechnology for biofuels and bioproducts · 2026Article
- The optimization of thermostable recombinant α-amylase for efficient fabric desizing using response surface methodology and surface morphology analysis.Bioprocess and biosystems engineering · 2026Article
- Overcoming cellular secretion bottlenecks: advanced secretion engineering and molecular tailoring for next-generation microbial α-amylases with enhanced industrial performance.World journal of microbiology & biotechnology · 2026Review
- Harnessing Bacillus velezensis for enhanced protease biosynthesis: optimization, purification, and kinetic profiling.BMC microbiology · 2026Article
- Complete genome sequence and functional characterization of Bacillus amyloliquefaciens NJF-55: a sheep-derived probiotic candidate.Scientific reports · 2026Article
- Purification and biochemical characterization of a novel thermostable acid α-amylase from Geotrichum candidum PO27 with industrial application.Folia microbiologica · 2025Article
- Deciphering the plant growth-promoting traits of bacteria capable of sodium dodecyl sulfate removal from graywater: a sustainable approach for water reuse for irrigation.Antonie van Leeuwenhoek · 2025Article
- Insight into the eminent biotechnological applications of xylanolytic enzymes for sustainable bioprocessing.3 Biotech · 2025Review
- Characterization of a Thermostable α-Amylase fromMicroorganisms · 2025Article
- Harnessing Waste Bread: From Potential Use in Microbial Growth and Enzyme Production to Techno-Economic Assessment.Microorganisms · 2025Article
- Optimization of thermostable amylolytic enzyme production from Bacillus cereus isolated from a recreational warm spring via Box Behnken design and response surface methodology.Microbial cell factories · 2025Article
- The role of statistical methods in optimizing and enhancing fungal chitosan commercial production.3 Biotech · 2025Review
- Article
- Optimization and characterization studies of poultry waste valorization for peptone production using a newly Egyptian Bacillus subtilis strain.AMB Express · 2025Article
- Study of Different Cultivated Plants Rhizosphere Soil Fungi-Mediated Pectinase: Insights into Production, Optimization, Purification, Biocompatibility, and Application.Microbial ecology · 2025Article
- CRISPR/Cas9-Engineering for Increased Amylolytic Potential of Microbes for Sustainable Wastewater Treatment: A Review.Current microbiology · 2024Review
- Bioremediation of metal cyanide complexes from electroplating wastewater for long-term application using3 Biotech · 2024Article
- Optimization, characterization and biosafety of carotenoids produced from whey using Micrococcus luteus.BMC biotechnology · 2024Article
- Optimization of newly isolated3 Biotech · 2024Article
Corrections and comments
- Erratum issued
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Food waste is a major issue, with one-third of food wasted yearly. This study aimed to produce sustainably the industrial enzyme alpha-amylase using discarded bread waste. Brown (BBW) and white bread waste (WBW) were tested as growth substrates using solid-state and submerged fermentation. The biosynthesized α- amylase is applied to treat starch-heavy industrial wastewater and for textile desizing. Bacillus amyloliquificiens showed the highest starch hydrolysis and enzyme activity on solid and liquid media. α-amylase production by B. amyloliquificiens was optimized via a one-factor-at-a-time evaluation of production parameters. Optimal production occurred by submerged fermentation of BBW inoculated with 2% B. amyloliquificiens at 37 °C and 200rpm for 24 h, reaching 695.2 U/mL α- amylase. The crude enzyme was immobilized on calcium alginate beads with 96.6% efficiency and kept 88.5% activity after 20 reuses, enhancing stability. A Box-Behnken design (BOX) assessed variable interactions. Response surface methodology (RSM) generated a quadratic model and analysis of variance (ANOVA analysis) fitting experimental starch hydrolysis data. Optimal conditions were pH 9, 45 °C, 70% starch, and 27.5 U/mL enzyme incubated for 15 min of contact time, with a high R
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