ArticleScientific reports2023
Rapid and sensitive detection of Pseudomonas aeruginosa by isothermal amplification combined with Cas12a-mediated detection.
Article in Scientific reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 17 citations in OpenAlex.
- The application of the CRISPR-Cas system in Pseudomonas aeruginosa infections.Molecular biology reports · 2026Review
- Development of an RT-RAA-CRISPR/Cas12a fluorescent detection method for the detection of bovine viral diarrhea virus 1.BMC veterinary research · 2026Article
- Garland Rolling Circle Amplification Mediated Self-Priming Extension Strategy for Sensitive and Label-FreeJournal of microbiology and biotechnology · 2026Article
- Development of a Strain-Specific Detection and Quantification Method forMicroorganisms · 2025Article
- Deciphering Multidrug-ResistantToxics · 2025Review
- CRISPR/Cas-Based Prenatal Screening for Aneuploidy: Challenges and Opportunities for Early Diagnosis.Medicina (Kaunas, Lithuania) · 2025Review
- Review
- Sensitive and Enzyme-FreeJournal of microbiology and biotechnology · 2024Article
- A Label-Free Fluorescent Amplification Strategy for High-Sensitive Detection ofJournal of microbiology and biotechnology · 2024Article
- An Evaluation of the Sensitivity and Applicability of a Droplet Digital Polymerase Chain Reaction Assay to Simultaneously DetectFoods (Basel, Switzerland) · 2024Article
- Rapid, sensitive, and user-friendly detection of Pseudomonas aeruginosa using the RPA/CRISPR/Cas12a system.BMC infectious diseases · 2024Article
- Rapid Visual Detection of African Swine Fever Virus with a CRISPR/Cas12a Lateral Flow Strip Based on Structural Protein Gene D117L.Animals : an open access journal from MDPI · 2023Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
CRISPR based technologies have been used for fast and sensitive detection of pathogens. To test the possibility of CRISPR based detection strategy in Pseudomonas aeruginosa infections, a combined method of recombinase polymerase amplification followed by Cas12a-mediated detection via fluorescence reader or lateral flow biosensor (named Cas12a-RCFL) has been established in this study. The Cas12a-RCFL can detect as low as 50 CFU/mL Pseudomonas aeruginosa. The whole detection process can be finished within one hour with satisfied detection specificity. Cas12a-RCFL also shows good sensitivity of detecting Pseudomonas aeruginosa inStaphylococcus aureus and Acinetobacter baumannii contaminated samples. For the detection of 22 clinical samples, Cas12a-RCFL matches with PCR sequencing result exactly without DNA purification. This Cas12a-RCFL is rapid and sensitive with low cost, which shows good quality to be adopted as a point-of-care testing method.
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Registered trials
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