Evidence map›Paper›PMID 37930553›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Quantifying Substrate Protein Secretion via the Type III Secretion System of the Bacterial Flagellum.

Rosa Einenkel, Manuel Halte, Marc Erhardt

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed, 1 pooled it
3.9field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 1 synthesis or guideline pooled it, 2 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. A catalog of proteins released fromMicrobiology spectrum · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Rosa EinenkelHumboldt Universität zu Berlin, Berlin, Germany.ORCID 0000-0002-6987-6225
Manuel HalteHumboldt Universität zu Berlin, Berlin, Germany.ORCID 0000-0001-9876-7493
Marc ErhardtHumboldt Universität zu Berlin, Berlin, Germany. marc.erhardt@hu-berlin.de.ORCID 0000-0001-6292-619X
Humboldt-Universität zu Berlin · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Protein transport across the cytoplasmic membrane is coupled to energy derived from ATP hydrolysis or the proton motive force. A sophisticated, multi-component type III secretion system (T3SS) exports substrate proteins of both the bacterial flagellum and virulence-associated injectisome system of many Gram-negative pathogens. The T3SS is primarily a proton motive force-driven protein exporter. Here, we describe a method to investigate the export of substrate proteins of the flagellar T3SS into the culture supernatant under conditions that manipulate the proton motive force. Further, we describe methods to precisely quantify flagellar protein export into the culture supernatant using a split NanoLuc luciferase, and how fluorescence labeling of the extracellular flagellar filament can bring insights into the protein export rate of individual flagellar T3SS.

Indexed as

FlagellaType III Secretion SystemsCell MembraneFluorescenceProtein TransportType III Secretion SystemsBacterial flagellumCarbonyl cyanide m-chlorophenylhydrazone (CCCP)Fluorescence microscopyIonophoreProtein exportProton motive forceSplit NanoLuc luciferaseT3SSType III secretion systemValinomycinΔpH gradientΔΨ gradient

Identifiers

PMID37930553
OpenAlexW4388422429

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.