Evidence map›Paper›PMID 37930545›Full record

ReviewMethods in molecular biology (Clifton, N.J.)2024

CryoEM Data Analysis of Membrane Proteins. Practical Considerations on Amphipathic Belts, Ligands, and Variability Analysis.

Alexia Gobet, Loïck Moissonnier, Vincent Chaptal

Abstract readReview
PubMed Publisher
In one paragraph

Review in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Alexia Gobet *Molecular Microbiology and Structural Biochemistry, UMR5086 CNRS University Lyon 1, Lyon, France.
Loïck Moissonnier *Molecular Microbiology and Structural Biochemistry, UMR5086 CNRS University Lyon 1, Lyon, France.
Vincent ChaptalMolecular Microbiology and Structural Biochemistry, UMR5086 CNRS University Lyon 1, Lyon, France. vincent.chaptal@ibcp.fr.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Membrane proteins data analysis by cryoEM shows some specificities, as can be found in other typical investigations such as biochemistry, biophysics, or X-ray crystallography. Membrane proteins are typically surrounded by an amphipathic belt that will have some degree of influence on the 3D reconstruction and analysis. In this chapter, we review our experience with the ABC transporter BmrA, as well as our statistical analysis of amphipathic belts around membrane proteins, to bring awareness on some particular features of membrane protein investigations by cryoEM.

Indexed as

ATP-Binding Cassette TransportersData AnalysisBiophysicsLigandsMembrane ProteinsATP-Binding Cassette TransportersLigandsMembrane ProteinsAmphipolsDetergentsElectron microscopyMembrane complexMembrane ProteinStructure

Identifiers

PMID37930545

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.