Evidence map›Paper›PMID 37929701›Full record

ArticleProteins2024

A structural analysis of the nsp9 protein from the coronavirus MERS CoV reveals a conserved RNA binding interface.

Gayathri Mani, Serene El-Kamand, Bing Wang, David L Baker, Sandro F Ataide, Irina Artsimovitch, Liza Cubeddu, Roland Gamsjaeger

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Proteins, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 46% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Gayathri ManiSchool of Science, Western Sydney University, Penrith, New South Wales, Australia.
Serene El-KamandSchool of Science, Western Sydney University, Penrith, New South Wales, Australia.
Bing WangDepartment of Microbiology, The Ohio State University, Columbus, Ohio, USA.
David L BakerSchool of Science, Western Sydney University, Penrith, New South Wales, Australia.
Sandro F AtaideSchool of Life and Environmental Sciences, University of Sydney, Camperdown, New South Wales, Australia.
Irina ArtsimovitchDepartment of Microbiology, The Ohio State University, Columbus, Ohio, USA.
Liza CubedduSchool of Science, Western Sydney University, Penrith, New South Wales, Australia.
Roland GamsjaegerSchool of Science, Western Sydney University, Penrith, New South Wales, Australia.ORCID 0000-0003-1095-2569
The University of Sydney · AUWestern Sydney University · AUThe Ohio State University · US

Funding

Mechnanism of transcript elongation control by RfaHR01GM067153 · NIGMS · OHIO STATE UNIVERSITY · PI IRINA ARTSIMOVITCH · 2003 to 2026
$8.0M
NIGMS NIH HHS R01 GM067153NIH HHS
6 · The paper itself

Abstract

Middle East respiratory syndrome coronavirus (MERS CoV) and severe acute respiratory syndrome coronavirus 2 (SARS CoV-2) are RNA viruses from the Betacoronavirus family that cause serious respiratory illness in humans. One of the conserved non-structural proteins encoded for by the coronavirus family is non-structural protein 9 (nsp9). Nsp9 plays an important role in the RNA capping process of the viral genome, where it is covalently linked to viral RNA (known as RNAylation) by the conserved viral polymerase, nsp12. Nsp9 also directly binds to RNA; we have recently revealed a distinct RNA recognition interface in the SARS CoV-2 nsp9 by using a combination of nuclear magnetic resonance spectroscopy and biolayer interferometry. In this study, we have utilized a similar methodology to determine a structural model of RNA binding of the related MERS CoV. Based on these data, we uncover important similarities and differences to SARS CoV-2 nsp9 and other coronavirus nsp9 proteins. Our findings that replacing key RNA binding residues in MERS CoV nsp9 affects RNAylation efficiency indicate that recognition of RNA may play a role in the capping process of the virus.

Indexed as

Middle East Respiratory Syndrome CoronavirusHumansRNASARS-CoV-2Viral Nonstructural ProteinsRNAViral Nonstructural ProteinscoronavirusCOVID-19MERS CoVnsp9RNASARS CoV-2

Identifiers

PMID37929701
PMCPMC10872591
OpenAlexW4388421492

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.