Evidence map›Paper›PMID 37929691›Full record

ArticleCurrent protocols2023

Cell Microencapsulation Within Engineered Hyaluronan Elastin-Like Protein (HELP) Hydrogels.

Meghan E Hefferon, Michelle S Huang, Yueming Liu, Renato S Navarro, Narelli de Paiva Narciso, Daiyao Zhang, Giselle Aviles-Rodriguez, Sarah C Heilshorn

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Current protocols, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Article
  2. Article
  3. Submucosal Hydrogel for Spring-Mediated Intestinal Lengthening.Journal of biomedical materials research. Part A · 2025
    Article
  4. Article
  5. Engineered Hydrogels for Organoid Models of Human Nonalcoholic Fatty Liver Disease.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025
    Article
  6. Engineered Protein Hydrogels as Biomimetic Cellular Scaffolds.Advanced materials (Deerfield Beach, Fla.) · 2024
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Meghan E HefferonDepartment of Materials Science and Engineering, Stanford University, Stanford, California, USA.ORCID https://orcid.org/0000-0002-6685-2121
Michelle S HuangDepartment of Chemical Engineering, Stanford University, Stanford, California, USA.ORCID https://orcid.org/0000-0002-1814-7786
Yueming LiuDepartment of Materials Science and Engineering, Stanford University, Stanford, California, USA.
Renato S NavarroDepartment of Materials Science and Engineering, Stanford University, Stanford, California, USA.ORCID https://orcid.org/0000-0001-5949-8251
Narelli de Paiva NarcisoDepartment of Materials Science and Engineering, Stanford University, Stanford, California, USA.ORCID https://orcid.org/0009-0002-9249-4644
Daiyao ZhangDepartment of Chemical Engineering, Stanford University, Stanford, California, USA.ORCID https://orcid.org/0009-0005-7259-0948
Giselle Aviles-RodriguezDepartment of Materials Science and Engineering, Stanford University, Stanford, California, USA.
Sarah C HeilshornDepartment of Materials Science and Engineering, Stanford University, Stanford, California, USA.ORCID https://orcid.org/0000-0002-9801-6304

Funding

Injectable Hydrogels to Deliver Gene Therapy for Myocardial InfarctR01HL151997 · NHLBI · STANFORD UNIVERSITY · PI HEILSHORN, SARAH C · 2020 to 2023
$1.7M
Engineered matrix microarrays to enhance the regenerative potential of iPSC-derived endothelial cellsR01HL142718 · NHLBI · STANFORD UNIVERSITY · PI HEILSHORN, SARAH C, HUANG, NGAN F. · 2018 to 2021
$1.6M
Engineered biomaterials to modulate cell-cell signaling for the robust expansion of stem cellsR01EB027171 · NIBIB · STANFORD UNIVERSITY · PI HEILSHORN, SARAH C · 2019 to 2022
$1.4M
An Engineered Hydrogel Platform to Improve Neural Organoid Reproducibility for a Multi-Organoid Disease Model of 22q11.2 Deletion SyndromeF31NS132505 · NINDS · STANFORD UNIVERSITY · PI HUANG, MICHELLE S · 2023 to 2025
$113k
NHLBI NIH HHS R01 HL142718NHLBI NIH HHS R01 HL151997NIBIB NIH HHS R01 EB027171NINDS NIH HHS F31 NS132505
6 · The paper itself

Abstract

Three-dimensional cell encapsulation has rendered itself a staple in the tissue engineering field. Using recombinantly engineered, biopolymer-based hydrogels to encapsulate cells is especially promising due to the enhanced control and tunability it affords. Here, we describe in detail the synthesis of our hyaluronan (i.e., hyaluronic acid) and elastin-like protein (HELP) hydrogel system. In addition to validating the efficacy of our synthetic process, we also demonstrate the modularity of the HELP system. Finally, we show that cells can be encapsulated within HELP gels over a range of stiffnesses, exhibit strong viability, and respond to stiffness cues. © 2023 Wiley Periodicals LLC. Basic Protocol 1: Elastin-like protein modification with hydrazine Basic Protocol 2: Nuclear magnetic resonance quantification of elastin-like protein modification with hydrazine Basic Protocol 3: Hyaluronic acid-benzaldehyde synthesis Basic Protocol 4: Nuclear magnetic resonance quantification of hyaluronic acid-benzaldehyde Basic Protocol 5: 3D cell encapsulation in hyaluronan elastin-like protein gels.

Indexed as

Hyaluronic AcidHydrogelsBenzaldehydesCell EncapsulationElastinHydrazinesBenzaldehydesElastinHyaluronic AcidHydrazinesHydrogelscell encapsulationdynamic covalent chemistryelastin-like proteinhyaluronanhydrogel

Identifiers

PMID37929691
PMCPMC10629846

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.