ArticleJournal of orthopaedic translation2023
A systematic investigation of the effects of TGF-β3 and mechanical stimulation on tenogenic differentiation of mesenchymal stromal cells in a poly(ethylene glycol)/gelatin-based hydrogel.
Article in Journal of orthopaedic translation, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 12 citations in OpenAlex.
- Dual-Responsive Hydrogels Engineer Anisotropic Cellular Microenvironment to Modulate Stem Cell Organization and Fate.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- SA/HA double-network hydrogel combined with PVDF electret membrane enhances osteogenic differentiation of DPSCs and promotes mandibular bone defect repair.Regenerative therapy · 2026Article
- Highly bio-adapted hydrogels for tendon-bone interface regeneration: Natural healing inspiration, design strategies, and biomedical potential.Bioactive materials · 2026Review
- Advanced bioactive materials and strategies for tendon repair and function restoration.Journal of orthopaedic translation · 2025Review
- Mechanical Reinforced and Self-healing Hydrogels: Bioprinted Biomimetic Methacrylated Collagen Peptide-Xanthan Gum Constructs for Ligament Regeneration.Advanced healthcare materials · 2025Article
- Aligned nanofiber scaffolds combined with cyclic stretch facilitate mesenchymal stem cell differentiation for ligament engineering.World journal of stem cells · 2025Article
- Biological tendon regeneration, are we almost there or is it still a stretch?Regenerative medicine · 2025Article
- Facile construction of mechanically robust and highly osteogenic materials for bone regeneration.Materials today. Bio · 2025Article
- Review
- Transcriptome-Optimized Hydrogel Design of a Stem Cell Niche for Enhanced Tendon Regeneration.Advanced materials (Deerfield Beach, Fla.) · 2025Article
- Assessing Bioprinted Functionalized Grafts for Biological Tendon Augmentation In Vitro.International journal of molecular sciences · 2024Article
- Repairing, reconstructing, and recovering after COVID-19.Journal of orthopaedic translation · 2023Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background: High post-surgical failure rates following tendon injury generate high medical costs and poor patient recovery. Cell-based tendon tissue engineering has the potential to produce fully functional replacement tissue and provide new strategies to restore tendon function and healing. In this endeavour, the application of mesenchymal stromal cells (MSCs) encapsulated in biomaterial scaffolds has shown great promise. However, a consensus on optimal promotion of tenogenic differentiation of MSCs has yet to be reached, although growth factors and mechanical cues are generally acknowledged as important factors. Methods: In this study, we prepared a hydrogel cell culture system consisting of methacrylated poly(d,l-lactic acid-ethylene glycol-d,l-lactic acid) (P(LA-EG-LA)) and gelatin methacrylate (GelMA) to encapsulate human bone marrow-derived MSCs (hBMSCs). We further systematically investigated the influence of static and intermittent cyclic uniaxial strain mechanical stimulation, in combination with transforming growth factor-β3 (TGF-β3) supplementation, on tenogenic differentiation of hBMSCs. Results: Increased TGF-β3 concentration upregulated the tenogenic genes Scleraxis ( Conclusion: Together, the most promising result for tenogenic differentiation of hBMSCs was identified as treatment with 5 ng/ml TGF-β3 under intermittent cyclic uniaxial strain (3% strain; 0.33 Hz; 1 h/day). This knowledge is of importance for the development of an improved protocol for tenogenic differentiation of MSCs and thereby for tendon tissue engineering. The translational potential of this article: Tissue-engineered strategies for tendon repair require a consensus on the differentiation of mesenchymal stromal cells to tenocytes, which is currently lacking. This article provides a systematic investigation of two main tenogenic differentiation conditions to further development of a tenogenic differentiation protocol.
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