Interobserver Variability in Semen Analysis: Findings From a Quality Control Initiative.

Introduction For laboratory tests, precision and accuracy are indispensable to ensure reliable results for both clinical diagnosis and research endeavors. The accuracy and reliability of results are important because they have an impact on both patient management and research. In this study, we evaluated the inter-observer variability between a trained technician and two academic residents, which acted both as a quality control measure as well as an assessment of training outcomes. Material and methods Freshly obtained semen samples from 28 subjects coming to the andrology laboratory were used. Semen analysis was performed by a regular technician permanently posted in the laboratory and two residents on completion of their posting in the andrology laboratory. All three examined the same sample after liquefaction for assessment of sperm motility, sperm concentration, sperm vitality, and sperm morphology. Semen analysis was done as per the recommendations of the WHO. Results The results of the study are presented as a coefficient of variation (CV), S charts, and Bland-Altman plot where we evaluated the interobserver variability in parameters on semen analysis of the same sample by three different assessors. The mean CV for sperm concentration across the samples was 6.24%. For sperm vitality, sperm morphology, and sperm motility the mean CV was 10.14%, 2.66%, and 8.11%, respectively. The S chart and Bland-Altman plot found a few random errors in measurements. Conclusion Regular quality control assessments are essential and should be implemented in andrology laboratories to ensure accurate and reliable results. Proper training of laboratory personnel is also vital for consistent outcomes. Other measures such as equipment calibration, use of high-quality reagents, and standard reporting are also crucial for the best results from a laboratory.