Evidence map›Paper›PMID 37927254›Full record

ArticleIn silico biology2023

scAN1.0: A reproducible and standardized pipeline for processing 10X single cell RNAseq data.

Maxime Lepetit, Mirela Diana Ilie, Marie Chanal, Gerald Raverot, Philippe Bertolino, Christophe Arpin, Franck Picard, Olivier Gandrillon

Abstract read
In one paragraph

Article in In silico biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Maxime LepetitENS de Lyon, CNRS UMR 5239, Laboratory of Biology and Modelling of the Cell, Lyon, France.
Mirela Diana IlieCentre de Recherche en Cancérologie (CRCL) - INSERM 1052 - CNRS 5286 - Centre Léon Bérard - Université Claude Bernard Lyon 1, Institut Convergence Plascan, Lyon, France.
Marie ChanalCentre de Recherche en Cancérologie (CRCL) - INSERM 1052 - CNRS 5286 - Centre Léon Bérard - Université Claude Bernard Lyon 1, Institut Convergence Plascan, Lyon, France.
Gerald RaverotCentre de Recherche en Cancérologie (CRCL) - INSERM 1052 - CNRS 5286 - Centre Léon Bérard - Université Claude Bernard Lyon 1, Institut Convergence Plascan, Lyon, France.
Philippe BertolinoCentre de Recherche en Cancérologie (CRCL) - INSERM 1052 - CNRS 5286 - Centre Léon Bérard - Université Claude Bernard Lyon 1, Institut Convergence Plascan, Lyon, France.
Christophe ArpinENS de Lyon, CNRS UMR 5239, Laboratory of Biology and Modelling of the Cell, Lyon, France.
Franck PicardENS de Lyon, CNRS UMR 5239, Laboratory of Biology and Modelling of the Cell, Lyon, France.
Olivier GandrillonENS de Lyon, CNRS UMR 5239, Laboratory of Biology and Modelling of the Cell, Lyon, France.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Single cell transcriptomics has recently seen a surge in popularity, leading to the need for data analysis pipelines that are reproducible, modular, and interoperable across different systems and institutions.To meet this demand, we introduce scAN1.0, a processing pipeline for analyzing 10X single cell RNA sequencing data. scAN1.0 is built using the Nextflow DSL2 and can be run on most computational systems. The modular design of Nextflow pipelines enables easy integration and evaluation of different blocks for specific analysis steps.We demonstrate the usefulness of scAN1.0 by showing its ability to examine the impact of the mapping step during the analysis of two datasets: (i) a 10X scRNAseq of a human pituitary gonadotroph tumor dataset and (ii) a murine 10X scRNAseq acquired on CD8 T cells during an immune response.

Indexed as

RNA-SeqSingle-Cell Gene Expression AnalysisSoftwareAnimalsCD8-Positive T-LymphocytesComputational BiologyDatasets as TopicGene Expression ProfilingHumansMicePituitary NeoplasmsWorkflowannotationdata analysismappingPipelinesingle cell transcriptomics

Identifiers

PMID37927254
PMCPMC10741331

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.