Evidence map›Paper›PMID 37926083›Full record

ArticlePathobiology : journal of immunopathology, molecular and cellular biology2024

Exploring the Expression of Adenosine Pathway-Related Markers CD73 and CD39 in Colorectal and Pancreatic Carcinomas Characterized by Multiplex Immunofluorescence: A Pilot Study.

Cibelle Freitas Lima, Auriole Tamegnon, Saxon Rodriguez, Dipen Maru, Philip L Martin, Zachary A Cooper, Jaime Rodriguez-Canales, Edwin Roger Parra

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Article in Pathobiology : journal of immunopathology, molecular and cellular biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Cibelle Freitas LimaDepartments of Translational Molecular Pathology, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA, cibellef09@gmail.com.
Auriole TamegnonDepartments of Translational Molecular Pathology, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA.
Saxon RodriguezDepartments of Translational Molecular Pathology, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA.
Dipen MaruDepartments of Anatomical Pathology, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA.
Philip L MartinDepartment of Translational Medicine, Oncology R&D, AstraZeneca, Gaithersburg, Maryland, USA.
Zachary A CooperDepartment of Translational Medicine, Oncology R&D, AstraZeneca, Gaithersburg, Maryland, USA.
Jaime Rodriguez-CanalesDepartment of Translational Medicine, Oncology R&D, AstraZeneca, Gaithersburg, Maryland, USA.
Edwin Roger ParraDepartments of Translational Molecular Pathology, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionGenerating high levels of immunosuppressive adenosine (ADO) in the tumor microenvironment contributes to cancer immune evasion. CD39 and CD73 hydrolyze adenosine triphosphate into ADO; thus, efforts have been made to target this pathway for cancer immunotherapy. Our objective was optimizing a multiplex immunofluorescence (mIF) panel to explore the role of CD39 and CD73 within the tumor microenvironment. MATERIALS AND

methodsIn three-time points, a small cohort (n = 8) of colorectal and pancreatic adenocarcinomas were automated staining using an mIF panel against CK, CD3, CD8, CD20, CD39, CD73, and CD68 to compare them with individual markers immunohistochemistry (IHC) for internal panel validation. Densities of immune cells and distances from different tumor-associated immune cells to tumor cells were exploratory assessment and compared with clinicopathologic variables and outcomes.

resultsComparing the three-time points and individual IHC staining results, we demonstrated high reproducibility of the mIF panel. CD39 and CD73 expression was low in malignant cells; the exploratory analysis showed higher densities of CD39 expression by various cells, predominantly stromal cells, followed by T cells, macrophages, and B cells. No expression of CD73 by B cells or macrophages was detected. Distance analysis revealed proximity of cytotoxic T cells, macrophages, and T cells expressing CD39 to malignant cells, suggesting a close regulatory signal driven by this ADO marker.

conclusionsWe optimized an mIF panel for detection of markers in the ADO pathway, an emerging clinically relevant pathway. The densities and spatial distribution demonstrated that this pathway may modulate aspects of the tumor immune microenvironment.

Indexed as

Antigens, CDBiomarkers, TumorColorectal NeoplasmsPancreatic Neoplasms5'-NucleotidaseAdenosineApyraseFluorescent Antibody Technique, DirectGPI-Linked ProteinsHumansPilot Projects5'-NucleotidaseAdenosineAntigens, CDApyraseBiomarkers, TumorCD39 antigenENTPD1 protein, humanGPI-Linked ProteinsNT5E protein, humanAdenosine pathwayCD39CD73Colorectal adenocarcinomaMultiplex immunofluorescencePancreatic ductal adenocarcinoma

Identifiers

PMID37926083
PMCPMC11524541

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.