Evidence map›Paper›PMID 37924815›Full record

ArticleCell reports methods2023

Generation of glucocorticoid-producing cells derived from human pluripotent stem cells.

Gerard Ruiz-Babot, Ariane Eceiza, Fernando Abollo-Jiménez, Maria Malyukov, Diana L Carlone, Kleiton Borges, Alexandra Rodrigues Da Costa, Shamma Qarin, Takuya Matsumoto, Ryuji Morizane and 7 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports methods, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed, 1 pooled it
2.0field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 1 synthesis or guideline pooled it, 13 citations in OpenAlex.

  1. Pooled it
  2. [Congenital adrenal hyperplasia].Problemy endokrinologii · 2026
    Review
  3. Review
  4. Review
  5. Article
  6. Utilization of Stem Cells in Medicine: A Narrative Review.International journal of molecular sciences · 2025
    Review
  7. Review
  8. Article
  9. Adrenocortical stem cells in health and disease.Nature reviews. Endocrinology · 2025
    Review
  10. Review
  11. Article
  12. Article
  13. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors at 9 institutions in 4 countries.

Gerard Ruiz-BabotDivision of Endocrinology, Boston Children's Hospital, Harvard Medical School, Boston, MA, USA; Harvard Stem Cell Institute, Cambridge, MA, USA; Department of Medicine, University Hospital Carl Gustav Carus, Dresden, Germany. Electronic address: gerard.ruiz-babot@uniklinikum-dresden.de.
Ariane EceizaDivision of Endocrinology, Boston Children's Hospital, Harvard Medical School, Boston, MA, USA; Harvard Stem Cell Institute, Cambridge, MA, USA.
Fernando Abollo-JiménezMaimonides Biomedical Research Institute of Cordoba (IMIBIC), Córdoba, Spain.
Maria MalyukovDepartment of Medicine, University Hospital Carl Gustav Carus, Dresden, Germany.
Diana L CarloneDivision of Endocrinology, Boston Children's Hospital, Harvard Medical School, Boston, MA, USA; Harvard Stem Cell Institute, Cambridge, MA, USA.
Kleiton BorgesDivision of Endocrinology, Boston Children's Hospital, Harvard Medical School, Boston, MA, USA; Harvard Stem Cell Institute, Cambridge, MA, USA.
Alexandra Rodrigues Da CostaCentre for Endocrinology, William Harvey Research Institute, Bart's and the London School of Medicine and Dentistry, Queen Mary University of London, London, UK.
Shamma QarinWellcome-MRC Cambridge Stem Cell Institute, Cambridge Biomedical Campus, University of Cambridge, Puddicombe Way, Cambridge, UK.
Takuya MatsumotoWyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, MA, USA; Nephrology Division, Massachusetts General Hospital, Boston, MA, USA.
Ryuji MorizaneHarvard Stem Cell Institute, Cambridge, MA, USA; Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, MA, USA; Nephrology Division, Massachusetts General Hospital, Boston, MA, USA.
William C SkarnesCellular Engineering, The Jackson Laboratory for Genomic Medicine, Farmington, CT 06032, USA.
Barbara LudwigDepartment of Medicine, University Hospital Carl Gustav Carus, Dresden, Germany.
Paul J ChappleCentre for Endocrinology, William Harvey Research Institute, Bart's and the London School of Medicine and Dentistry, Queen Mary University of London, London, UK.
Leonardo GuastiCentre for Endocrinology, William Harvey Research Institute, Bart's and the London School of Medicine and Dentistry, Queen Mary University of London, London, UK.
Helen L StorrCentre for Endocrinology, William Harvey Research Institute, Bart's and the London School of Medicine and Dentistry, Queen Mary University of London, London, UK.
Stefan R BornsteinDepartment of Medicine, University Hospital Carl Gustav Carus, Dresden, Germany; Division of Endocrinology, Diabetes and Nutritional Sciences, Faculty of Life Sciences and Medicine, King's College London, London, UK.
David T BreaultDivision of Endocrinology, Boston Children's Hospital, Harvard Medical School, Boston, MA, USA; Harvard Stem Cell Institute, Cambridge, MA, USA. Electronic address: david.breault@childrens.harvard.edu.
Harvard University · USQueen Mary University of London · GBUniversity Hospital Carl Gustav Carus · DEBoston Children's Hospital · USHarvard Stem Cell Institute · USInstituto Maimónides de Investigación Biomédica de Córdoba · ESJackson Laboratory · USUniversity of Cambridge · GBWilliam Harvey Research Institute · GB

Funding

Recreating Kidney Organogenesis in vitro with Human Pluripotent Stem CellsDP2DK133821 · NIDDK · MASSACHUSETTS GENERAL HOSPITAL · PI MORIZANE, RYUJI · 2019 to 2019
$2.5M
Medical Research Council MR/P019897/1
6 · The paper itself

Abstract

Adrenal insufficiency is a life-threatening condition resulting from the inability to produce adrenal hormones in a dose- and time-dependent manner. Establishing a cell-based therapy would provide a physiologically responsive approach for the treatment of this condition. We report the generation of large numbers of human-induced steroidogenic cells (hiSCs) from human pluripotent stem cells (hPSCs). Directed differentiation of hPSCs into hiSCs recapitulates the initial stages of human adrenal development. Following expression of steroidogenic factor 1, activation of protein kinase A signaling drives a steroidogenic gene expression profile most comparable to human fetal adrenal cells, and leads to dynamic secretion of steroid hormones, in vitro. Moreover, expression of the adrenocorticotrophic hormone (ACTH) receptor/co-receptor (MC2R/MRAP) results in dose-dependent ACTH responsiveness. This protocol recapitulates adrenal insufficiency resulting from loss-of-function mutations in AAAS, which cause the enigmatic triple A syndrome. Our differentiation protocol generates sufficient numbers of hiSCs for cell-based therapy and offers a platform to study disorders causing adrenal insufficiency.

Indexed as

Adrenal InsufficiencyPluripotent Stem CellsAdrenocorticotropic HormoneEsophageal AchalasiaGlucocorticoidsHumansReceptors, CorticotropinAdrenocorticotropic HormoneGlucocorticoidsReceptors, CorticotropinACTHadrenal insufficiencyadrenal steroidallgrove syndromecell therapyCP: Stem cellhuman pluripotent stem cellssteroidogenesistriple A syndrome

Identifiers

PMID37924815
PMCPMC10694497
OpenAlexW4388289236

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.