Evidence map›Paper›PMID 37924215›Full record

ArticlePlant & cell physiology2024

Glutathione Transferase Photoaffinity Labeling Displays GST Induction by Safeners and Pathogen Infection.

Maria Font Farre, Daniel Brown, Maurice König, Brian J Killinger, Farnusch Kaschani, Markus Kaiser, Aaron T Wright, Jonathan Burton, Renier A L van der Hoorn

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant & cell physiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.8field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 5 citations in OpenAlex.

  1. Herbicidal safening activity ofFrontiers in plant science · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 3 countries.

Maria Font FarreThe Plant Chemetics Laboratory, Department of Biology, University of Oxford, Oxford OX1 3RB, UK.
Daniel BrownChemistry Research Laboratory, Department of Chemistry, University of Oxford, Oxford, Oxfordshire OX1 3TA, UK.
Maurice KönigThe Plant Chemetics Laboratory, Department of Biology, University of Oxford, Oxford OX1 3RB, UK.
Brian J KillingerVoiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, WA 99164, USA.
Farnusch KaschaniZMB Chemical Biology, Faculty of Biology, University of Duisburg-Essen, Essen 45141, Germany.
Markus KaiserZMB Chemical Biology, Faculty of Biology, University of Duisburg-Essen, Essen 45141, Germany.
Aaron T WrightVoiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, WA 99164, USA.
Jonathan BurtonChemistry Research Laboratory, Department of Chemistry, University of Oxford, Oxford, Oxfordshire OX1 3TA, UK.
Renier A L van der HoornThe Plant Chemetics Laboratory, Department of Biology, University of Oxford, Oxford OX1 3RB, UK.
University of Oxford · GBUniversity of Duisburg-Essen · DEBaylor University · USWashington State University · US

Funding

Biotechnology and Biological Sciences Research Council DDT00060
6 · The paper itself

Abstract

Glutathione transferases (GSTs) represent a large and diverse enzyme family involved in the detoxification of small molecules by glutathione conjugation in crops, weeds and model plants. In this study, we introduce an easy and quick assay for photoaffinity labeling of GSTs to study GSTs globally in various plant species. The small-molecule probe contains glutathione, a photoreactive group and a minitag for coupling to reporter tags via click chemistry. Under UV irradiation, this probe quickly and robustly labels GSTs in crude protein extracts of different plant species. Purification and mass spectrometry (MS) analysis of labeled proteins from Arabidopsis identified 10 enriched GSTs from the Phi(F) and Tau(U) classes. Photoaffinity labeling of GSTs demonstrated GST induction in wheat seedlings upon treatment with safeners and in Arabidopsis leaves upon infection with avirulent bacteria. Treatment of Arabidopsis with salicylic acid (SA) analog benzothiadiazole (BTH) induces GST labeling independent of NPR1, the master regulator of SA. Six Phi- and Tau-class GSTs that are induced upon BTH treatment were identified, and their labeling was confirmed upon transient overexpression. These data demonstrate that GST photoaffinity labeling is a useful approach to studying GST induction in crude extracts of different plant species upon different types of stress.

Indexed as

ArabidopsisArabidopsis ProteinsGlutathioneGlutathione TransferaseSalicylic AcidArabidopsis ProteinsGlutathioneGlutathione TransferaseSalicylic AcidAgrochemicalChemical proteomicsGlutathione transferaseGSTImmunityPhotoaffinity labelingSafener

Identifiers

PMID37924215
PMCPMC10799724
OpenAlexW4388342203

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.