Evidence map›Paper›PMID 37924095›Full record

ArticleBMC biotechnology2023

Preparation, purification, and biochemical of fat-degrading bacterial enzymes from pig carcass compost and its application.

Xinran Duan, Wei Zhai, Xintian Li, Sicheng Wu, Ye Wang, Lixia Wang, Wangdui Basang, Yanbin Zhu, Yunhang Gao

Open access · goldAbstract read
In one paragraph

Article in BMC biotechnology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.1field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 7 citations in OpenAlex.

  1. A ProbioticMicroorganisms · 2026
    Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Xinran DuanCollege of Veterinary Medicine, Jilin Agricultural University, Changchun, 130118, China.
Wei ZhaiCollege of Veterinary Medicine, Jilin Agricultural University, Changchun, 130118, China.
Xintian LiCollege of Veterinary Medicine, Jilin Agricultural University, Changchun, 130118, China.
Sicheng WuCollege of Veterinary Medicine, Jilin Agricultural University, Changchun, 130118, China.
Ye WangCollege of Veterinary Medicine, Jilin Agricultural University, Changchun, 130118, China.
Lixia WangNortheast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, 130102, China.
Wangdui BasangInstitute of Animal Husbandry and Veterinary Medicine, Tibet Academy of Agricultural and Animal Husbandry Science, Lhasa, 850009, China.
Yanbin ZhuInstitute of Animal Husbandry and Veterinary Medicine, Tibet Academy of Agricultural and Animal Husbandry Science, Lhasa, 850009, China.
Yunhang GaoCollege of Veterinary Medicine, Jilin Agricultural University, Changchun, 130118, China. gaoyunhang@163.com.
Jilin Agricultural University · CNAnimal Science Research Institute · CNChinese Academy of Sciences · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundA lot of kitchen waste oil is produced every day worldwide, leading to serious environmental pollution. As one of the environmental protection methods, microorganisms are widely used treating of various wastes. Lipase, as one of the cleaning agents can effectively degrade kitchen waste oil. The composting process of pig carcasses produces many lipase producing microorganisms, rendering compost products an excellent source for isolating lipase producing microorganisms. To our knowledge, there are no reports isolating of lipase producing strains from the high temperature phase of pig carcass compost. METHODOLOGY: Lipase producing strains were isolated using a triglyceride medium and identified by 16S rRNA gene sequencing. The optimal fermentation conditions for maximum lipase yield were gradually optimized by single-factor tests. The extracellular lipase was purified by ammonium sulfate precipitation and Sephadex G-75 gel isolation chromatography. Amino acid sequence analysis, structure prediction, and molecular docking of the purified protein were performed. The pure lipase's enzymatic properties and application potential were evaluated by characterizing its biochemical properties.

resultsIn this study, a lipase producing strain of Bacillus sp. ZF2 was isolated from pig carcass compost products, the optimal fermentation conditions of lipase: sucrose 3 g/L, ammonium sulfate 7 g/L, Mn

conclusionsThis study reveals the potential of lipase for waste oil removal, and future research will be devoted to the application of lipase.

Indexed as

CompostingAmmonium SulfateAnimalsHydrogen-Ion ConcentrationLipaseMolecular Docking SimulationRNA, Ribosomal, 16SSwineTemperatureAmmonium SulfateLipaseRNA, Ribosomal, 16SBacillus sp.CharacterizationLipaseOptimizationPurification

Identifiers

PMID37924095
PMCPMC10625193
OpenAlexW4388290752

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.