ArticleScientific reports2023
Detection and characterization of putative hypervirulent Klebsiella pneumoniae isolates in microbiological diagnostics.
Article in Scientific reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 2 of them syntheses that pooled it.
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Who cites it
31 citing papers in PubMed, 2 syntheses or guidelines pooled it, 27 citations in OpenAlex.
- A systematic review of hypervirulentFrontiers in medicine · 2025Pooled it
- Pooled it
- Clinical and genomic profiling ofVirulence · 2026Article
- Effects of bovine lactoferrin on the growth and biofilm formation of Escherichia coli and Klebsiella pneumoniae associated with bovine mastitis.Veterinary research communications · 2026Article
- Molecular characterization of carbapenem resistance and hypervirulence determinants of Klebsiella pneumoniae circulating in a tertiary hospital in Cyprus.Molecular biology reports · 2026Article
- Tet-ON and Tet-OFF regulation in hypervirulentMicrobiology spectrum · 2026Article
- Genomic, virulent and phenotypic characterization of a cerebrospinal fluid-derived ST86-KL2 hypervirulent Klebsiella pneumoniae isolate from a patient with meningitis and diabetes mellitus.BMC microbiology · 2026Article
- Emerging hypervirulent and extensively drug-resistant Klebsiella pneumoniae with potent biofilm formation as a new clinical challenge.Molecular biology reports · 2026Article
- Interplay Between Virulence Genes, Antimicrobial Resistance Profiles, and Biofilm Formation in Klebsiella pneumoniae Causing Urinary Tract Infections in North Lebanon.MicrobiologyOpen · 2026Article
- Genomic epidemiology of putative hypervirulentMicrobiology spectrum · 2026Article
- Identification of candidate nucleomodulins in ESKAPE bacteria -Frontiers in cellular and infection microbiology · 2026Article
- Emergence and genomic characterization of hypervirulent ST23/K1Frontiers in microbiology · 2026Article
- Suspected community-acquisition of carbapenem-resistant hypervirulent Klebsiella pneumoniae (CRhvKp) in Germany: a case report and implications for infection control.GMS hygiene and infection control · 2026Article
- The dual threat of hypervirulent and multidrug-resistantFrontiers in microbiology · 2026Review
- Retrospective single-center study of endogenous endophthalmitis, Northeastern China, 2010-2023.Frontiers in cellular and infection microbiology · 2026Article
- The promising activity of apple cider vinegar on MDR Klebsiella spp. (K. variicola and K. pneumoniae) emerging pathogens in chicken.Veterinary research communications · 2025Article
- Effect of lactoferrin on biofilm formation of bovine mastitis-causing Staphylococcus species.The Journal of veterinary medical science · 2025Article
- Epidemiology and genomic characteristics of carbapenem-resistant Klebsiella pneumoniae in intensive care unit from 2021 to 2024.BMC microbiology · 2025Article
- Fatal Pneumocephalus Caused by Hypervirulent Klebsiella pneumoniae, Germany.Emerging infectious diseases · 2025Article
- Cross-border prevalence of putative-hypervirulentNew microbes and new infections · 2025Article
Corrections and comments
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Authors and funding
13 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hypervirulent Klebsiella pneumoniae strains (hvKp) can cause invasive community-acquired infections in healthy patients of all ages. In this study, the prevalence of putative hvKp in a German tertiary center was investigated and hvKp were characterized by phenotypic and molecular assays. All K. pneumoniae isolates in routine microbiological diagnostics from a single center were screened by string-testing over a period of 6 months. String-test positive (≥ 0.5 mm) isolates were re-evaluated on different media and under various conditions (aerobe, anaerobe). For string-test positive isolates, genes (magA, iutA, rmpA and rmpA2) associated with hypermucoviscosity and hypervirulence were amplified by multiplex PCR. PCR-positive isolates were subjected to whole-genome sequencing and sedimentation and biofilm formation assays. From 1310 screened K. pneumoniae isolates in clinical routine 100 isolates (7.6%) were string test positive. From these, 9% (n = 9) were defined as putative hvKp (string-test+/PCR+). Highest rate of string-test-positive isolates was observed on MacConkey agar under aerobic conditions. Amongst these nine putative hvKp isolates, the international lineage ST23 carrying hvKp-plasmid pKpVP-1 was the most common, but also a rare ST86 with pKpVP-2 was identified. All nine isolates showed hypermucoviscosity and weak biofilm formation. In conclusion, 9% of string-positive, respectively 0.69% of all K. pneumoniae isolates from routine were defined as putative hypervirulent. MacConkey agar was the best medium for hvKp screening.
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