Evidence map›Paper›PMID 37919959›Full record

ArticleJournal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc2024

Normalizing real-time PCR results in routine testing.

Betsy Armenta-Leyva, Berenice Munguía-Ramírez, Ting-Yu Cheng, Fangshu Ye, Alexandra Henao-Díaz, Luis G Giménez-Lirola, Jeffrey Zimmerman

Abstract read
In one paragraph

Article in Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Betsy Armenta-LeyvaDepartment of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Lloyd Veterinary Medical Center, Iowa State University, Ames, IA, USA.ORCID 0000-0003-4094-6813
Berenice Munguía-RamírezDepartment of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Lloyd Veterinary Medical Center, Iowa State University, Ames, IA, USA.ORCID 0000-0002-2557-1817
Ting-Yu ChengDepartment of Veterinary Preventive Medicine, College of Veterinary Medicine, the Ohio State University, Columbus, OH, USA.ORCID 0000-0002-3732-9147
Fangshu YeDepartment of Statistics, College of Liberal Arts and Sciences, Iowa State University, Ames, IA, USA.ORCID 0000-0001-6994-1210
Alexandra Henao-DíazPig Improvement México, Santiago de Querétaro, Querétaro, México.
Luis G Giménez-LirolaDepartment of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Lloyd Veterinary Medical Center, Iowa State University, Ames, IA, USA.ORCID 0000-0002-4407-7996
Jeffrey ZimmermanDepartment of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Lloyd Veterinary Medical Center, Iowa State University, Ames, IA, USA.ORCID 0000-0002-8107-2422

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Normalization, the process of controlling for normal variation in sampling and testing, can be achieved in real-time PCR assays by converting sample quantification cycles (Cqs) to "efficiency standardized Cqs" (ECqs). We calculated ECqs as E

Indexed as

Porcine Reproductive and Respiratory SyndromePorcine respiratory and reproductive syndrome virusSwine DiseasesViral VaccinesAnimalsAntibodies, ViralQuinoxalinesReal-Time Polymerase Chain ReactionSulfanilamidesSwineVaccines, Attenuated5-chloroquinoxaline-2-sulfanilamideAntibodies, ViralQuinoxalinesSulfanilamidesVaccines, AttenuatedViral Vaccinesamplification efficiencyECqnormalizationreal-time PCRrun-to-run variation

Identifiers

PMID37919959
PMCPMC10734596

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.