ArticleNature methods2023
Reconstruction of dynamic mammary mini gland in vitro for normal physiology and oncogenesis.
Article in Nature methods, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 34 citations in OpenAlex.
- Beyond H5N1: influenza A virus infection in bovine udder organoids.Emerging microbes & infections · 2026Article
- Construction and application of mammary and breast cancer organoids.Cell insight · 2026Review
- Establishing a long-term versatile culture system for functional mouse mammary organoids from single cells.Nature protocols · 2026Review
- Advances in vascularized organoids.Chinese medical journal · 2026Review
- Unravelling genome-wide mosaic microsatellite mutations at single-cell resolution.bioRxiv : the preprint server for biology · 2026Article
- Reconstitution of spermatogenesis and continuous generation of functional haploid germ cells in mouse testicular organoids.Nature communications · 2026Article
- Article
- Developing Mammary Gland Models for Biomedical Applications.Research (Washington, D.C.) · 2026Review
- Biomimetic Mammary Gland Organoid-on-a-Chip for Producing Selected Human Milk Components.Research (Washington, D.C.) · 2026Article
- 3D Nanofibrillar Matrix Stiffness Modulates Extracellular Vesicle Cargo and Pro-Tumour Functions.Journal of extracellular vesicles · 2025Article
- Accelerated maturation of branched organoids confined in collagen droplets.Lab on a chip · 2025Article
- Volumetric printed biomimetic scaffolds support in vitro lactation of human milk-derived mammary epithelial cells.Science advances · 2025Article
- Mucinous breast cancer organoids: an in vitro research model.Discover oncology · 2025Article
- Rational Design and Organoid-Based Evaluation of a Cocktail CAR-γδ T Cell Therapy for Heterogeneous Glioblastoma.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Distinct mammary stem cells orchestrate long-term homeostasis of adult mammary gland.Cell discovery · 2025Article
- Review
- The role of extracellular matrix viscoelasticity in development and disease.npj biological physics and mechanics · 2025Review
- Magnetically Actuated Soft Electrodes for Multisite Bioelectrical Monitoring of Ex Vivo Tissues.Cyborg and bionic systems (Washington, D.C.) · 2025Article
- Emerging strategies to investigate the biology of early cancer.Nature reviews. Cancer · 2024Review
- Establishment of goat mammary organoid cultures modeling the mammary gland development and lactation.Journal of animal science and biotechnology · 2024Article
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Organoid culture has been extensively exploited for normal tissue reconstruction and disease modeling. However, it is still challenging to establish organoids that mimic in vivo-like architecture, size and function under homeostatic conditions. Here we describe the development of a long-term adult stem cell-derived mammary mini gland culture system that supports robust three-dimensional outgrowths recapitulating the morphology, scale, cellular context and transcriptional heterogeneity of the normal mammary gland. The self-organization ability of stem cells and the stability of the outgrowths were determined by a coordinated combination of extracellular matrix, environmental signals and dynamic physiological cycles. We show that these mini glands were hormone responsive and could recapitulate the entire postnatal mammary development including puberty, estrus cycle, lactation and involution. We also observed that these mini glands maintained the presence of mammary stem cells and could also recapitulate the fate transition from embryonic bipotency to postnatal unipotency in lineage tracing assays. In addition, upon induction of oncogene expression in the mini glands, we observed tumor initiation in vitro and in vivo in a mouse model. Together, this study provides an experimental system that can support a dynamic miniature mammary gland for the study of physiologically relevant, complex biological processes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.