Evidence map›Paper›PMID 37919265›Full record

ArticleNature communications2023

In situ quantification of osmotic pressure within living embryonic tissues.

Antoine Vian, Marie Pochitaloff, Shuo-Ting Yen, Sangwoo Kim, Jennifer Pollock, Yucen Liu, Ellen M Sletten, Otger Campàs

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.

0numbers the graph read from it
0cells of the map it votes in
30citing papers in PubMed
4.6field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

30 citing papers in PubMed, 42 citations in OpenAlex.

  1. Biophysics of lumen morphogenesis.Development (Cambridge, England) · 2026
    Review
  2. Mechanics of compression-driven morphogenesis.Development (Cambridge, England) · 2026
    Review
  3. Article
  4. Article
  5. Review
  6. Article
  7. Article
  8. Review
  9. Article
  10. Article
  11. Design and optimization of in situ self-functionalizing stress sensors.The European physical journal. E, Soft matter · 2026
    Article
  12. Article
  13. Review
  14. Article
  15. Review
  16. Article
  17. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Antoine VianDepartment of Mechanical Engineering, University of California, Santa Barbara, CA, USA.ORCID 0000-0001-8774-320X
Marie PochitaloffDepartment of Mechanical Engineering, University of California, Santa Barbara, CA, USA.ORCID 0000-0001-9264-8127
Shuo-Ting YenDepartment of Mechanical Engineering, University of California, Santa Barbara, CA, USA.
Sangwoo KimDepartment of Mechanical Engineering, University of California, Santa Barbara, CA, USA.
Jennifer PollockDepartment of Mechanical Engineering, University of California, Santa Barbara, CA, USA.
Yucen LiuDepartment of Mechanical Engineering, University of California, Santa Barbara, CA, USA.
Ellen M SlettenDepartment of Chemistry and Biochemistry, University of California, Los Angeles, CA, USA.ORCID 0000-0002-0049-7278
Otger CampàsDepartment of Mechanical Engineering, University of California, Santa Barbara, CA, USA. otger.campas@tu-dresden.de.
University of California, Santa Barbara · USUniversity of California, Los Angeles · US

Funding

Robust microdroplet-based mechanical probes for wide-ranging mechanobiology applicationsR01GM135380 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI CAMPAS, OTGER, SLETTEN, ELLEN MAY · 2019 to 2022
$1.8M
NIGMS NIH HHS R01 GM135380
6 · The paper itself

Abstract

Mechanics is known to play a fundamental role in many cellular and developmental processes. Beyond active forces and material properties, osmotic pressure is believed to control essential cell and tissue characteristics. However, it remains very challenging to perform in situ and in vivo measurements of osmotic pressure. Here we introduce double emulsion droplet sensors that enable local measurements of osmotic pressure intra- and extra-cellularly within 3D multicellular systems, including living tissues. After generating and calibrating the sensors, we measure the osmotic pressure in blastomeres of early zebrafish embryos as well as in the interstitial fluid between the cells of the blastula by monitoring the size of droplets previously inserted in the embryo. Our results show a balance between intracellular and interstitial osmotic pressures, with values of approximately 0.7 MPa, but a large pressure imbalance between the inside and outside of the embryo. The ability to measure osmotic pressure in 3D multicellular systems, including developing embryos and organoids, will help improve our understanding of its role in fundamental biological processes.

Indexed as

Extracellular FluidZebrafishAnimalsEmbryo, MammalianEmulsionsOsmotic PressureEmulsions

Identifiers

PMID37919265
PMCPMC10622550
OpenAlexW4388217741

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.