Evidence map›Paper›PMID 37914453›Full record

ArticleMethods in enzymology2023

Structure-seq of tRNAs and other short RNAs in droplets and in vivo.

McCauley O Meyer, Saehyun Choi, Christine D Keating, Philip C Bevilacqua, Ryota Yamagami

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in enzymology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
3.3field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 4 citations in OpenAlex.

  1. Optimized tRNA structure-seq reveals robust tRNA secondary structures inbioRxiv : the preprint server for biology · 2026
    Article
  2. tRNA pseudouridine synthase D (TruD) fromRNA (New York, N.Y.) · 2025
    Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 2 countries.

McCauley O MeyerDepartment of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA, United States; Center for RNA Molecular Biology, Pennsylvania State University, University Park, PA, United States.
Saehyun ChoiDepartment of Chemistry, Pennsylvania State University, University Park, PA, United States; Department of Chemistry, University of California, Berkeley, CA, United States.
Christine D KeatingDepartment of Chemistry, Pennsylvania State University, University Park, PA, United States. Electronic address: keating@chem.psu.edu.
Philip C BevilacquaDepartment of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA, United States; Center for RNA Molecular Biology, Pennsylvania State University, University Park, PA, United States; Department of Chemistry, Pennsylvania State University, University Park, PA, United States. Electronic address: pcb5@psu.edu.
Ryota YamagamiDepartment of Materials Science and Biotechnology, Graduate School of Science and Engineering, Ehime University, Matsuyama, Ehime, Japan.
Pennsylvania State University · USEhime University · JPUniversity of California, Berkeley · US

Funding

RNA folding and catalysis at the interface of biophysics and genomicsR35GM127064 · NIGMS · PENNSYLVANIA STATE UNIVERSITY, THE · PI PHILIP C BEVILACQUA · 2018 to 2026
$3.4M
NIGMS NIH HHS R35 GM127064
6 · The paper itself

Abstract

There is a multitude of small (<100nt) RNAs that serve diverse functional roles in biology. Key amongst these is transfer RNA (tRNA), which is among the most ancient RNAs and is part of the translational apparatus in every domain of life. Transfer RNAs are also the most heavily modified class of RNAs. They are essential and their misregulation, due to mutated sequences or loss of modification, can lead to disease. Because of the severe phenotypes associated with mitochondrial tRNA defects in particular, the desire to deliver repaired tRNAs via droplets such as lipid nanoparticles or other compartments is an active area of research. Here we describe how to use our tRNA Structure-seq method to study tRNAs and other small RNAs in two different biologically relevant contexts, peptide-rich droplets and in vivo.

Indexed as

RNARNA, TransferRNARNA, TransferChemical probingIn-droplet RNA structurein vivo RNA structureMutational profilingRNA modificationsSmall RNAsTRNA

Identifiers

PMID37914453
PMCPMC10917389
OpenAlexW4382286842

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.