Evidence map›Paper›PMID 37906309›Full record

ArticleEnvironmental monitoring and assessment2023

The H9c2(2-1) cell-based sulforhodamine B assay is a non-animal alternative to evaluate municipal wastewater quality over time.

Elsa T Rodrigues, Eduarda Pereira, Paulo J Oliveira, Miguel A Pardal

Open access · hybridAbstract read
In one paragraph

Article in Environmental monitoring and assessment, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.2field-weighted citation impact, top 57% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 3 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 3 institutions in 1 country.

Elsa T RodriguesCentre for Functional Ecology, Associate Laboratory TERRA, Department of Life Sciences, University of Coimbra, Calçada Martim de Freitas, 3000-456, Coimbra, Portugal. etrodrig@uc.pt.ORCID https://orcid.org/0000-0002-9541-7890
Eduarda PereiraDepartment of Chemistry and CESAM/REQUIMTE, University of Aveiro, Campus de Santiago, 3810-193, Aveiro, Portugal.ORCID https://orcid.org/0000-0002-6046-5243
Paulo J OliveiraCenter for Neuroscience and Cell Biology, UC Biotech, University of Coimbra, Biocant Park, 3060-197, Cantanhede, Portugal.ORCID https://orcid.org/0000-0002-5201-9948
Miguel A PardalCentre for Functional Ecology, Associate Laboratory TERRA, Department of Life Sciences, University of Coimbra, Calçada Martim de Freitas, 3000-456, Coimbra, Portugal.ORCID https://orcid.org/0000-0001-6048-7007
University of Coimbra · PTBiocant · PTRede de Química e Tecnologia · PT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The present study validates the potential of the in vitro H9c2(2-1) cell-based sulforhodamine B (SRB) assay to evaluate the temporal variability of wastewater quality. The impact of effluent disposal on water quality and the efficiency of the wastewater treatment process were also assessed. To correlate standard analytical method results with in vitro results, a total of 16 physicochemical parameters, such as nutrients, pH, chemical oxygen demand, total suspended solids and metals, were determined in both raw and treated wastewater samples. Results revealed that the H9c2(2-1) cell-based SRB assay has an enormous potential to evaluate municipal wastewater quality over time and to discriminate influent and effluent toxic characteristics, as well as for water quality monitoring and surveillance of the efficacy of treatment processes. Finally, the gathered results alerted to the impact of phosphates in a biological system, leading us to recommend the selection of this parameter as a potential environmental health indicator.

Indexed as

Water Pollutants, ChemicalWater PurificationBiological Oxygen Demand AnalysisEnvironmental MonitoringRhodaminesWaste Disposal, FluidWastewaterlissamine rhodamine BRhodaminesWastewaterWater Pollutants, ChemicalEffluent toxicity testingEnvironmental biomonitoringEnvironmental health indicatorsH9c2(2-1) cellsSulforhodamine B assayTemporal variability

Identifiers

PMID37906309
PMCPMC10618365
OpenAlexW4388046672

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.