Evidence map›Paper›PMID 37905143›Full record

ArticlebioRxiv : the preprint server for biology2023

A blue-shifted genetically encoded Ca

Abhi Aggarwal, Smrithi Sunil, Imane Bendifallah, Michael Moon, Mikhail Drobizhev, Landon Zarowny, Jihong Zheng, Sheng-Yi Wu, Alexander W Lohman, Alison G Tebo and 4 more

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

14 authors at 6 institutions in 4 countries.

Abhi AggarwalUniversity of Alberta, Department of Chemistry, Edmonton, Alberta, Canada.
Smrithi SunilAllen Institute for Neural Dynamics, Seattle, Washington, United States of America.
Imane BendifallahVision Institute, Sorbonne University, CNRS, INSERM, Paris, France.
Michael MoonUniversity of Alberta, Department of Chemistry, Edmonton, Alberta, Canada.
Mikhail DrobizhevMontana State University, Department of Microbiology and Cell Biology, Bozeman, Montana, United States of America.
Landon ZarownyUniversity of Alberta, Department of Chemistry, Edmonton, Alberta, Canada.
Jihong ZhengHoward Hughes Medical Institute, Janelia Research Campus, Ashburn, Virginia, United States of America.
Sheng-Yi WuUniversity of Alberta, Department of Chemistry, Edmonton, Alberta, Canada.
Alexander W LohmanUniversity of Calgary, Department of Cell Biology and Anatomy and Hotchkiss Brain Institute, Calgary, Alberta, Canada.
Alison G TeboHoward Hughes Medical Institute, Janelia Research Campus, Ashburn, Virginia, United States of America.
Valentina EmilianiVision Institute, Sorbonne University, CNRS, INSERM, Paris, France.
Kaspar PodgorskiAllen Institute for Neural Dynamics, Seattle, Washington, United States of America.
Yi ShenUniversity of Alberta, Department of Chemistry, Edmonton, Alberta, Canada.
Robert E CampbellUniversity of Alberta, Department of Chemistry, Edmonton, Alberta, Canada.
University of Alberta · CAHoward Hughes Medical Institute · USCentre National de la Recherche Scientifique · FRAllen Institute · USMontana State University · USUniversity of Calgary · CA

Funding

Resource for Multiphoton Characterization of Genetically-Encoded ProbesU24NS109107 · NINDS · MONTANA STATE UNIVERSITY - BOZEMAN · PI Mikhail Drobizhev · 2018 to 2026
$2.2M
NINDS NIH HHS U24 NS109107
6 · The paper itself

Abstract

Significance: Genetically encoded calcium ion (Ca Aim: We describe the development and applications of T-GECO1 - a high-performance blue-shifted GECI based on the Approach: We used protein engineering and extensive directed evolution to develop T-GECO1. We characterize the purified protein and assess its performance Results: The Ca Conclusion: T-GECO1 is a high performance blue-shifted GECI that, under two-photon excitation conditions, provides advantages relative to late generation GCaMP variants.

Indexed as

blue-shifted fluorescencegenetically encoded calcium ion indicatorneuronal activity imagingprotein engineeringtwo-photon excitation

Identifiers

PMID37905143
PMCPMC10614751
OpenAlexW4387730038

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.