Evidence map›Paper›PMID 37905093›Full record

ArticlebioRxiv : the preprint server for biology2023

Mechanical coupling coordinates microtubule growth.

Bonnibelle K Leeds, Katelyn F Kostello, Yuna Y Liu, Christian R Nelson, Sue Biggins, Charles L Asbury

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Bonnibelle K LeedsPhysiology & Biophysics Department, University of Washington School of Medicine, Seattle WA, USA.
Katelyn F KostelloPhysiology & Biophysics Department, University of Washington School of Medicine, Seattle WA, USA.
Yuna Y LiuPhysiology & Biophysics Department, University of Washington School of Medicine, Seattle WA, USA.
Christian R NelsonFred Hutchinson Cancer Research Center.
Sue BigginsFred Hutchinson Cancer Research Center.
Charles L AsburyPhysiology & Biophysics Department, University of Washington School of Medicine, Seattle WA, USA.
University of Washington · USFred Hutch Cancer Center · US

Funding

Translational Bioimaging Core Shared ResourceP30CA015704 · NCI · FRED HUTCHINSON CANCER RESEARCH CENTER · PI Eric Collisson · 1985 to 2026
$296.4M
TRAINING IN MOLECULAR BIOPHYSICST32GM008268 · NIGMS · UNIVERSITY OF WASHINGTON · PI KOLLMAN, JUSTIN M, ZHENG, NING · 1988 to 2023
$8.6M
The Yeast Centrosome - Structure Assembly & FunctionP01GM105537 · NIGMS · UNIVERSITY OF COLORADO · PI DAVIS, TRISHA N. · 2014 to 2018
$8.3M
Regulation of Chromosome SegregationR01GM064386 · NIGMS · FRED HUTCHINSON CANCER RESEARCH CENTER · PI BIGGINS, SUSAN · 2002 to 2022
$7.2M
Reconstitution and biophysical study of chromosome segregation machineryR35GM134842 · NIGMS · UNIVERSITY OF WASHINGTON · PI Charles L Asbury · 2020 to 2026
$4.7M
Supplement to Biophysical Study of Reconstituted Kinetochore-microtubule AttachmentsR01GM079373 · NIGMS · UNIVERSITY OF WASHINGTON · PI ASBURY, CHARLES · 2006 to 2019
$4.5M
Multicolor TIRF microscope for studying mitotic spindle components at the singleS10RR026406 · NCRR · UNIVERSITY OF WASHINGTON · PI ASBURY, CHARLES · 2010 to 2010
$214k
NCI NIH HHS P30 CA015704NCRR NIH HHS S10 RR026406NIGMS NIH HHS P01 GM105537NIGMS NIH HHS R01 GM064386NIGMS NIH HHS R01 GM079373NIGMS NIH HHS R35 GM134842NIGMS NIH HHS T32 GM008268
6 · The paper itself

Abstract

During mitosis, kinetochore-attached microtubules form bundles (k-fibers) in which many filaments grow and shorten in near-perfect unison to align and segregate each chromosome. However, individual microtubules grow at intrinsically variable rates, which must be tightly regulated for a k-fiber to behave as a single unit. This exquisite coordination might be achieved biochemically, via selective binding of polymerases and depolymerases, or mechanically, because k-fiber microtubules are coupled through a shared load that influences their growth. Here, we use a novel dual laser trap assay to show that microtubule pairs growing

Identifiers

PMID37905093
PMCPMC10614740
OpenAlexW4382931295

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.