Evidence map›Paper›PMID 37905023›Full record

ArticlebioRxiv : the preprint server for biology2023

Multi-pass, single-molecule nanopore reading of long protein strands with single-amino acid sensitivity.

Keisuke Motone, Daphne Kontogiorgos-Heintz, Jasmine Wee, Kyoko Kurihara, Sangbeom Yang, Gwendolin Roote, Yishu Fang, Nicolas Cardozo, Jeff Nivala

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 15 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 2 countries.

Keisuke MotonePaul. G. Allen School of Computer Science and Engineering, University of Washington, Seattle, WA, USA.
Daphne Kontogiorgos-HeintzPaul. G. Allen School of Computer Science and Engineering, University of Washington, Seattle, WA, USA.
Jasmine WeePaul. G. Allen School of Computer Science and Engineering, University of Washington, Seattle, WA, USA.
Kyoko KuriharaPaul. G. Allen School of Computer Science and Engineering, University of Washington, Seattle, WA, USA.
Sangbeom YangPaul. G. Allen School of Computer Science and Engineering, University of Washington, Seattle, WA, USA.
Gwendolin RootePaul. G. Allen School of Computer Science and Engineering, University of Washington, Seattle, WA, USA.
Yishu FangPaul. G. Allen School of Computer Science and Engineering, University of Washington, Seattle, WA, USA.
Nicolas CardozoMolecular Engineering and Science Institute, University of Washington, Seattle, WA, USA.
Jeff NivalaPaul. G. Allen School of Computer Science and Engineering, University of Washington, Seattle, WA, USA.
University of Washington · USDiponegoro University · ID

Funding

Long-read single-molecule protein sequencing on an array of unfoldase-coupled nanoporesR01HG012545 · NHGRI · UNIVERSITY OF WASHINGTON · PI NIVALA, JEFFREY MATTHEW · 2022 to 2024
$1.8M
NHGRI NIH HHS R01 HG012545
6 · The paper itself

Abstract

The ability to sequence single protein molecules in their native, full-length form would enable a more comprehensive understanding of proteomic diversity. Current technologies, however, are limited in achieving this goal. Here, we establish a method for long-range, single-molecule reading of intact protein strands on a commercial nanopore sensor array. By using the ClpX unfoldase to ratchet proteins through a CsgG nanopore, we achieve single-amino acid level sensitivity, enabling sequencing of combinations of amino acid substitutions across long protein strands. For greater sequencing accuracy, we demonstrate the ability to reread individual protein molecules, spanning hundreds of amino acids in length, multiple times, and explore the potential for high accuracy protein barcode sequencing. Further, we develop a biophysical model that can simulate raw nanopore signals

Identifiers

PMID37905023
PMCPMC10614977
OpenAlexW4387813660

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.