Evidence map›Paper›PMID 37904871›Full record

ArticleMagnetic resonance (Gottingen, Germany)2022

Site-selective generation of lanthanoid binding sites on proteins using 4-fluoro-2,6-dicyanopyridine.

Sreelakshmi Mekkattu Tharayil, Mithun C Mahawaththa, Akiva Feintuch, Ansis Maleckis, Sven Ullrich, Richard Morewood, Michael J Maxwell, Thomas Huber, Christoph Nitsche, Daniella Goldfarb and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Magnetic resonance (Gottingen, Germany), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
2.6field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 11 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 3 countries.

Sreelakshmi Mekkattu TharayilResearch School of Chemistry, Australian National University, Canberra, ACT 2601, Australia.
Mithun C MahawaththaARC Centre of Excellence for Innovations in Peptide & Protein Science, Research School of Chemistry, Australian National University, Canberra, ACT 2601, Australia.
Akiva FeintuchDepartment of Chemical Physics, Weizmann Institute of Science, Rehovot 76100, Israel.
Ansis MaleckisLatvian Institute of Organic Synthesis, Aizkraukles 21, 1006 Riga, Latvia.
Sven UllrichResearch School of Chemistry, Australian National University, Canberra, ACT 2601, Australia.
Richard MorewoodResearch School of Chemistry, Australian National University, Canberra, ACT 2601, Australia.
Michael J MaxwellARC Centre of Excellence for Innovations in Peptide & Protein Science, Research School of Chemistry, Australian National University, Canberra, ACT 2601, Australia.
Thomas HuberResearch School of Chemistry, Australian National University, Canberra, ACT 2601, Australia.
Christoph NitscheResearch School of Chemistry, Australian National University, Canberra, ACT 2601, Australia.
Daniella GoldfarbDepartment of Chemical Physics, Weizmann Institute of Science, Rehovot 76100, Israel.
Gottfried OttingARC Centre of Excellence for Innovations in Peptide & Protein Science, Research School of Chemistry, Australian National University, Canberra, ACT 2601, Australia.
Australian National University · AUWeizmann Institute of Science · ILLatvijas Organiskās Sintēzes Institūts · LV

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The paramagnetism of a lanthanoid tag site-specifically installed on a protein provides a rich source of structural information accessible by nuclear magnetic resonance (NMR) and electron paramagnetic resonance (EPR) spectroscopy. Here we report a lanthanoid tag for selective reaction with cysteine or selenocysteine with formation of a (seleno)thioether bond and a short tether between the lanthanoid ion and the protein backbone. The tag is assembled on the protein in three steps, comprising (i) reaction with 4-fluoro-2,6-dicyanopyridine (FDCP); (ii) reaction of the cyano groups with

Identifiers

PMID37904871
PMCPMC10539774
OpenAlexW4295350421

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.