Evidence map›Paper›PMID 37904765›Full record

ArticleMagnetic resonance (Gottingen, Germany)2021

Towards resolving the complex paramagnetic nuclear magnetic resonance (NMR) spectrum of small laccase: assignments of resonances to residue-specific nuclei.

Rubin Dasgupta, Karthick B S S Gupta, Huub J M de Groot, Marcellus Ubbink

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Magnetic resonance (Gottingen, Germany), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Rubin DasguptaLeiden Institute of Chemistry, University of Leiden, Gorlaeus Laboratory, Einsteinweg 55, 2333 CC, Leiden, the Netherlands.
Karthick B S S GuptaLeiden Institute of Chemistry, University of Leiden, Gorlaeus Laboratory, Einsteinweg 55, 2333 CC, Leiden, the Netherlands.
Huub J M de GrootLeiden Institute of Chemistry, University of Leiden, Gorlaeus Laboratory, Einsteinweg 55, 2333 CC, Leiden, the Netherlands.
Marcellus UbbinkLeiden Institute of Chemistry, University of Leiden, Gorlaeus Laboratory, Einsteinweg 55, 2333 CC, Leiden, the Netherlands.
Leiden University · NL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Laccases efficiently reduce dioxygen to water in an active site containing a tri-nuclear copper centre (TNC). The dynamics of the protein matrix is a determining factor in the efficiency in catalysis. To probe mobility, nuclear magnetic resonance (NMR) spectroscopy is highly suitable. However, several factors complicate the assignment of resonances to active site nuclei in laccases. The paramagnetic nature causes large shifts and line broadening. Furthermore, the presence of slow chemical exchange processes of the imidazole rings of copper ligand results in peak doubling. A third complicating factor is that the enzyme occurs in two states, the native intermediate (NI) and resting oxidized (RO) states, with different paramagnetic properties. The present study aims at resolving the complex paramagnetic NMR spectra of the TNC of

Identifiers

PMID37904765
PMCPMC10539750
OpenAlexW3128591576

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.