Evidence map›Paper›PMID 37902188›Full record

ArticleDisease models & mechanisms2023

Episodic live imaging of cone photoreceptor maturation in GNAT2-EGFP retinal organoids.

Jinlun Bai, David S Koos, Kayla Stepanian, Zachary Fouladian, Dominic W H Shayler, Jennifer G Aparicio, Scott E Fraser, Rex A Moats, David Cobrinik

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Disease models & mechanisms, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.6field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
  4. Article
  5. Article
  6. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Jinlun BaiThe Vision Center, Department of Surgery, Children's Hospital Los Angeles, Los Angeles, CA 90027, USA.
David S KoosThe Saban Research Institute, Children's Hospital Los Angeles, Los Angeles, CA 90027, USA.
Kayla StepanianThe Vision Center, Department of Surgery, Children's Hospital Los Angeles, Los Angeles, CA 90027, USA.
Zachary FouladianThe Vision Center, Department of Surgery, Children's Hospital Los Angeles, Los Angeles, CA 90027, USA.
Dominic W H ShaylerThe Vision Center, Department of Surgery, Children's Hospital Los Angeles, Los Angeles, CA 90027, USA.
Jennifer G AparicioThe Vision Center, Department of Surgery, Children's Hospital Los Angeles, Los Angeles, CA 90027, USA.
Scott E FraserThe Saban Research Institute, Children's Hospital Los Angeles, Los Angeles, CA 90027, USA.
Rex A MoatsThe Saban Research Institute, Children's Hospital Los Angeles, Los Angeles, CA 90027, USA.
David CobrinikThe Vision Center, Department of Surgery, Children's Hospital Los Angeles, Los Angeles, CA 90027, USA.ORCID 0000-0002-4478-2417
University of Southern California · USChildren's Hospital of Los Angeles · US

Funding

Successive responses to oncogenic aberrations in retinoblastoma genesis.R01CA137124 · NCI · SLOAN-KETTERING INST CAN RESEARCH · PI David Cobrinik · 2011 to 2026
$6.3M
Training in Developmental Biology, Stem Cells and RegenerationT32HD060549 · NICHD · UNIVERSITY OF SOUTHERN CALIFORNIA · PI Gage D Crump · 2011 to 2026
$2.5M
Human Specific Signaling Circuitry in Cone Precursor DevelopmentR01EY026661 · NEI · CHILDREN'S HOSPITAL OF LOS ANGELES · PI COBRINIK, DAVID · 2016 to 2019
$1.7M
NCI NIH HHS R01 CA137124NEI NIH HHS R01 EY026661NICHD NIH HHS T32 HD060549NIH HHS 5R01CA137124NIH HHS 5T32HD060549NIH HHS R01EY026661
6 · The paper itself

Abstract

Fluorescent reporter pluripotent stem cell-derived retinal organoids are powerful tools to investigate cell type-specific development and disease phenotypes. When combined with live imaging, they enable direct and repeated observation of cell behaviors within a developing retinal tissue. Here, we generated a human cone photoreceptor reporter line by CRISPR/Cas9 genome editing of WTC11-mTagRFPT-LMNB1 human induced pluripotent stem cells (iPSCs) by inserting enhanced green fluorescent protein (EGFP) coding sequences and a 2A self-cleaving peptide at the N-terminus of guanine nucleotide-binding protein subunit alpha transducin 2 (GNAT2). In retinal organoids generated from these iPSCs, the GNAT2-EGFP alleles robustly and exclusively labeled immature and mature cones. Episodic confocal live imaging of hydrogel immobilized retinal organoids allowed tracking of the morphological maturation of individual cones for >18 weeks and revealed inner segment accumulation of mitochondria and growth at 12.2 μm3 per day from day 126 to day 153. Immobilized GNAT2-EGFP cone reporter organoids provide a valuable tool for investigating human cone development and disease.

Indexed as

Induced Pluripotent Stem CellsRetinal Cone Photoreceptor CellsCell DifferentiationGreen Fluorescent ProteinsHumansOrganoidsRetinaenhanced green fluorescent proteinGreen Fluorescent ProteinsCone photoreceptorCRISPRFluorescent reporterGNAT2iPSCLive imagingRetinal organoid

Identifiers

PMID37902188
PMCPMC10690052
OpenAlexW4388014628

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.