Evidence map›Paper›PMID 37901204›Full record

ArticleFrontiers in immunology2023

Using combined single-cell gene expression, TCR sequencing and cell surface protein barcoding to characterize and track CD4+ T cell clones from murine tissues.

Annekathrin Silvia Nedwed, Sara Salome Helbich, Kathrin Luise Braband, Michael Volkmar, Michael Delacher, Federico Marini

Abstract read
In one paragraph

Article in Frontiers in immunology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Annekathrin Silvia NedwedInstitute of Medical Biostatistics, Epidemiology and Informatics (IMBEI), University Medical Center Mainz, Mainz, Germany.
Sara Salome HelbichInstitute of Immunology, University Medical Center Mainz, Mainz, Germany.
Kathrin Luise BrabandInstitute of Immunology, University Medical Center Mainz, Mainz, Germany.
Michael VolkmarHelmholtz-Institute for Translational Oncology Mainz (HI-TRON Mainz), Mainz, Germany.
Michael DelacherInstitute of Immunology, University Medical Center Mainz, Mainz, Germany.
Federico MariniInstitute of Medical Biostatistics, Epidemiology and Informatics (IMBEI), University Medical Center Mainz, Mainz, Germany.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Single-cell gene expression analysis using sequencing (scRNA-seq) has gained increased attention in the past decades for studying cellular transcriptional programs and their heterogeneity in an unbiased manner, and novel protocols allow the simultaneous measurement of gene expression, T-cell receptor clonality and cell surface protein expression. In this article, we describe the methods to isolate scRNA/TCR-seq-compatible CD4

Indexed as

CD4-Positive T-LymphocytesSoftwareAnimalsClone CellsGene ExpressionMembrane ProteinsMiceReceptors, Antigen, T-CellMembrane ProteinsReceptors, Antigen, T-CellCD4 T cellscRNA seqscTCR seqTCR (T-cell receptor)tissue CD4 T cell

Identifiers

PMID37901204
PMCPMC10602882

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.