Evidence map›Paper›PMID 37900763›Full record

ArticleFrontiers in plant science2023

An accurate, reliable, and universal qPCR method to identify homozygous single insert T-DNA with the example of transgenic rice.

Hai Thanh Tran, Carly Schramm, My-My Huynh, Yuri Shavrukov, James C R Stangoulis, Colin L D Jenkins, Peter A Anderson

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in plant science, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.6field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 4 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Copy number variations at theFrontiers in plant science · 2024
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Hai Thanh TranCollege of Science and Engineering, Flinders University, Adelaide, SA, Australia.
Carly SchrammCollege of Science and Engineering, Flinders University, Adelaide, SA, Australia.
My-My HuynhCollege of Science and Engineering, Flinders University, Adelaide, SA, Australia.
Yuri ShavrukovCollege of Science and Engineering, Flinders University, Adelaide, SA, Australia.
James C R StangoulisCollege of Science and Engineering, Flinders University, Adelaide, SA, Australia.
Colin L D JenkinsCollege of Science and Engineering, Flinders University, Adelaide, SA, Australia.
Peter A AndersonCollege of Science and Engineering, Flinders University, Adelaide, SA, Australia.
Flinders University · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Early determination of transgenic plants that are homozygous for a single locus T-DNA insert is highly desirable in most fundamental and applied transgenic research. This study aimed to build on an accurate, rapid, and reliable quantitative real-time PCR (qPCR) method to fast-track the development of multiple homozygous transgenic rice lines in the T

Indexed as

copy numberhomozygous plantNOS terminatorOsSBE4 reference genequantitative real-time PCRsingle T-DNAtransgenic ricezygosity identification

Identifiers

PMID37900763
PMCPMC10600460
OpenAlexW4387514115

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.