ArticleNature communications2023
Wastewater sequencing reveals community and variant dynamics of the collective human virome.
Article in Nature communications, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers, 2 of them syntheses that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
59 citing papers in PubMed, 2 syntheses or guidelines pooled it, 118 citations in OpenAlex.
- Wastewater Surveillance for Early Warning of Infectious Disease Outbreaks: A Systematic Review of Evidence and Implications for One Health Surveillance.Pathogens (Basel, Switzerland) · 2026Pooled it
- Methodological Approaches to Dengue Virus Detection in Wastewater: A Systematic Review and Meta-Analysis of Positivity Rate.Viruses · 2026Pooled it
- Review
- Human rotavirus C strain detected in wastewater in Leuven, Belgium.Microbiology resource announcements · 2026Article
- Future-use consent and human virome research: ethical challenges.Journal of virology · 2026Review
- Temporal Alignment of Wastewater Signals with Clinical Indicators of Respiratory Illness Postpandemic, Texas, USA.Emerging infectious diseases · 2026Article
- Comparative Analysis of Viral Communities in Hospital, University and Urban Wastewater by Shotgun Metagenomic Sequencing.International journal of molecular sciences · 2026Article
- Empowering One Health with metagenomics.One health outlook · 2026Review
- Detection, persistence, and rising prevalence of oncogenic viruses revealed by wastewater metagenomics.Applied and environmental microbiology · 2026Article
- Genomic wastewater surveillance of human and animal influenza A viruses in California during the 2024-2025 flu season.medRxiv : the preprint server for health sciences · 2026Article
- Statewide multi-year wastewater sequencing reveals dual origins of HIV-1 signal.Nature communications · 2026Article
- Detection of Viruses of Public Health Importance in Wastewater Samples Using Conventional PCR Techniques and a Targeted Enrichment Whole Genome Sequencing Panel.Food and environmental virology · 2026Article
- Article
- Transkingdom Interactions Between Viruses and Bacteria: Implications for Wastewater Treatment Efficiency.Food and environmental virology · 2026Review
- Gut virome-microbiome interactions across hosts and environments.NPJ biofilms and microbiomes · 2026Article
- Fecal virome at the human-animal interface: a one health perspective on an uncharted frontier.Animal microbiome · 2026Review
- Tools and approaches to study the human gut virome: from the bench to bioinformatics.mSystems · 2026Review
- WEPP: Phylogenetic placement achieves near-haplotype resolution in wastewater-based epidemiology.PLoS computational biology · 2026Article
- Metagenomic sequencing identifies potential respiratory pathogens in PCR-negative subset of surveillance samples.Scientific reports · 2026Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
30 authors at 3 institutions in 2 countries.
Funding
Abstract
Wastewater is a discarded human by-product, but its analysis may help us understand the health of populations. Epidemiologists first analyzed wastewater to track outbreaks of poliovirus decades ago, but so-called wastewater-based epidemiology was reinvigorated to monitor SARS-CoV-2 levels while bypassing the difficulties and pit falls of individual testing. Current approaches overlook the activity of most human viruses and preclude a deeper understanding of human virome community dynamics. Here, we conduct a comprehensive sequencing-based analysis of 363 longitudinal wastewater samples from ten distinct sites in two major cities. Critical to detection is the use of a viral probe capture set targeting thousands of viral species or variants. Over 450 distinct pathogenic viruses from 28 viral families are observed, most of which have never been detected in such samples. Sequencing reads of established pathogens and emerging viruses correlate to clinical data sets of SARS-CoV-2, influenza virus, and monkeypox viruses, outlining the public health utility of this approach. Viral communities are tightly organized by space and time. Finally, the most abundant human viruses yield sequence variant information consistent with regional spread and evolution. We reveal the viral landscape of human wastewater and its potential to improve our understanding of outbreaks, transmission, and its effects on overall population health.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.