ArticleAnimals : an open access journal from MDPI2023
Temperature Elevation during Semen Delivery Deteriorates Boar Sperm Quality by Promoting Apoptosis.
Article in Animals : an open access journal from MDPI, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 11 citations in OpenAlex.
- Dietary Arginine Supplementation Mitigates Heat Stress-Induced Testicular Dysfunction Through Arginine-Related Metabolic Remodeling and Autophagy-Associated Stress Adaptation in a Rongchang Boar Model.Animals : an open access journal from MDPI · 2026Article
- Impact of cold storage on semen quality in two divergent rabbit lines selected for resilience under heat stress condition.Veterinary and animal science · 2026Article
- Effects of Heat Stress on Semen Quality and Reproductive Performance of Crossbred Boars with Different Maternal Origins.Animals : an open access journal from MDPI · 2026Article
- Nanoparticle-Based Approaches for Enhancing In Vitro Fertilization in Animal Reproduction.International journal of molecular sciences · 2026Review
- N-salicyloyl tryptamine derivative ameliorates spermatogenic dysfunction in obese mice by attenuating insulin resistance, enhancing Sertoli cell glycolysis, and inhibiting apoptosis: anFrontiers in cell and developmental biology · 2026Article
- Reproductive performance of a semi-automated insemination system technology in sows.Animal reproduction · 2026Article
- Pre-cooling of boar semen before transport in a hot environment enhances biosafety.Frontiers in microbiology · 2025Article
- CMOS-fabricated dielectrophoretic chip with embedded 3D TiN nano-electrode arrays for sperm capture and sperm damage reduction.Frontiers in bioengineering and biotechnology · 2025Article
- Innovative CMOS-fabricated dielectrophoretic chip: application of 3D TiN nano-electrode arrays with adjustable electrode spacing in sperm capture.Frontiers in bioengineering and biotechnology · 2025Article
- Supplementing Boar Diet with Nicotinamide Mononucleotide Improves Sperm Quality Probably through the Activation of the SIRT3 Signaling Pathway.Antioxidants (Basel, Switzerland) · 2024Article
- Sperm quality andFrontiers in veterinary science · 2024Article
- Post-Thaw Storage Temperature Influenced Boar Sperm Quality and Lifespan through Apoptosis and Lipid Peroxidation.Animals : an open access journal from MDPI · 2023Article
Corrections and comments
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Authors and funding
9 authors at 5 institutions in 4 countries.
Funding
Abstract
Semen delivery practice is crucial to the efficiency of artificial insemination using high-quality boar sperm. The present study aimed to evaluate the effect of a common semen delivery method, a Styrofoam box, under elevated temperatures on boar sperm quality and functionality and to investigate the underlying molecular responses of sperm to the temperature rise. Three pooled semen samples from 10 Duroc boars (3 ejaculates per boar) were used in this study. Each pooled semen sample was divided into two aliquots. One aliquot was stored at a constant 17 °C as the control group. Another one was packaged in a well-sealed Styrofoam box and placed in an incubator at 37 °C for 24 h to simulate semen delivery on hot summer days and subsequently transferred to a refrigerator at 17 °C for 3 days. The semen temperature was continuously monitored. The semen temperature was 17 °C at 0 h of storage and reached 20 °C at 5 h, 30 °C at 14 h, and 37 °C at 24 h. For each time point, sperm quality and functionality, apoptotic changes, expression levels of phosphorylated AMPK, and heat shock proteins HSP70 and HSP90 were determined by CASA, flow cytometry, and Western blotting. The results showed that elevated temperature during delivery significantly deteriorated boar sperm quality and functionality after 14 h of delivery. Storage back to 17 °C did not recover sperm motility. An increased temperature during delivery apparently promoted the conversion of sperm early apoptosis to late apoptosis, showing a significant increase in the expression levels of Bax and Caspase 3. The levels of phosphorylated AMPK were greatly induced by the temperature rise to 20 °C during delivery but reduced thereafter. With the temperature elevation, expression levels of HSP70 and HSP90 were notably increased. Our results indicate that a temperature increase during semen delivery greatly damages sperm quality and functionality by promoting sperm apoptosis. HSP70 and HSP90 could participate in boar sperm resistance to temperature changes by being associated with AMPK activation and anti-apoptotic processes.
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Registered trials
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