Evidence map›Paper›PMID 37892920›Full record

ArticleBioengineering (Basel, Switzerland)2023

Investigation of DNA Hybridization on Nano-Structured Plasmonic Surfaces for Identifying Nasopharyngeal Viruses.

Shao-Sian Li, Yi-Jung Lu, Ray Chang, Ming-Han Tsai, Jo-Ning Hung, Wei-Hung Chen, Yu-Jui Fan, Pei-Kuen Wei, Horn-Jiunn Sheen

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bioengineering (Basel, Switzerland), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.6field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 3 citations in OpenAlex.

  1. Review
  2. Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 6 institutions in 1 country.

Shao-Sian LiDepartment of Materials and Mineral Resources, National Taipei University of Technology, Taipei 10608, Taiwan.
Yi-Jung LuDivision of Family and Operative Dentistry, Department of Dentistry, Taipei Medical University Hospital, Taipei 11031, Taiwan.
Ray ChangInstitute of Applied Mechanics, National Taiwan University, No. 1, Section 4, Roosevelt Rd, Taipei 10617, Taiwan.
Ming-Han TsaiInstitute of Microbiology & Immunology, National Yang Ming Chiao Tung University, No. 155, Section 2, Linong St., Beitou District, Taipei 11221, Taiwan.
Jo-Ning HungInstitute of Microbiology & Immunology, National Yang Ming Chiao Tung University, No. 155, Section 2, Linong St., Beitou District, Taipei 11221, Taiwan.
Wei-Hung ChenInstitute of Applied Mechanics, National Taiwan University, No. 1, Section 4, Roosevelt Rd, Taipei 10617, Taiwan.
Yu-Jui FanSchool of Biomedical Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei 11031, Taiwan.
Pei-Kuen WeiResearch Center for Applied Sciences, Academia Sinica, 128 Academia Road, Section 2, Nankang, Taipei 11529, Taiwan.
Horn-Jiunn SheenInstitute of Applied Mechanics, National Taiwan University, No. 1, Section 4, Roosevelt Rd, Taipei 10617, Taiwan.ORCID 0000-0001-8971-9019
National Taiwan University · TWNational Yang Ming Chiao Tung University · TWAcademia Sinica · TWNational Taipei University of Technology · TWTaipei Medical University · TWTaipei Medical University Hospital · TW

Funding

joint project of Academia Sinica and National Taiwan University AS-NTU-110-07National Science and Technology Council, Taiwan 111-2221-E-002-128National Science and Technology Council, Taiwan 111-2636-E-038-004National Science and Technology Council, Taiwan 111-2811-E-002-030
6 · The paper itself

Abstract

Recently, studies have revealed that human herpesvirus 4 (HHV-4), also known as the Epstein-Barr virus, might be associated with the severity of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Compared to SARS-CoV-2 infection alone, patients coinfected with SARS-CoV-2 and HHV-4 had higher risks of fever, inflammation, and even death, thus, confirming that HHV-4/SARS-CoV-2 coinfection in patients could benefit from clinical investigation. Although several intelligent devices can simultaneously discern multiple genes related to SARS-CoV-2, most operate via label-based detection, which restricts them from directly measuring the product. In this study, we developed a device that can replicate and detect SARS-CoV-2 and HHV-4 DNA. This device can conduct a duplex polymerase chain reaction (PCR) in a microfluidic channel and detect replicates in a non-labeled manner through a plasmonic-based sensor. Compared to traditional instruments, this device can reduce the required PCR time by 55% while yielding a similar amount of amplicon. Moreover, our device's limit of detection (LOD) reached 100 fg/mL, while prior non-labeled sensors for SARS-CoV-2 detection were in the range of ng/mL to pg/mL. Furthermore, the device can detect desired genes by extracting cells artificially infected with HHV-4/SARS-CoV-2. We expect that this device will be able to help verify HHV-4/SARS-CoV-2 coinfected patients and assist in the evaluation of practical treatment approaches.

Indexed as

duplex PCRhuman herpesvirus 4label-freenanoslit-based plasmonicspolymerase chain reactionSARS-CoV-2

Identifiers

PMID37892920
PMCPMC10604513
OpenAlexW4387614185

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.