Evidence map›Paper›PMID 37892706›Full record

ArticleJournal of clinical medicine2023

Normothermic Ex Situ Machine Perfusion of Vascularized Composite Allografts with Oxygen Microcarriers for 12 Hours Using Real-Time Mitochondrial Redox Quantification.

Valentin Haug, Yifeng Peng, Bianief Tchiloemba, Alice T Wang, Florian Buerger, Padraic Romfh, Ulrich Kneser, Brian D Polizzotti, Bohdan Pomahac

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of clinical medicine, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.3field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 6 institutions in 3 countries.

Valentin HaugDivision of Plastic Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.ORCID 0000-0002-2964-979X
Yifeng PengDepartment of Cardiology, Boston Children's Hospital, Harvard Medical School, Boston, MA 02115, USA.
Bianief TchiloembaDivision of Plastic Surgery, Department of Surgery, University of Calgary, Calgary, AB T2N 4N1, Canada.ORCID 0000-0002-0408-8113
Alice T WangDivision of Plastic Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.ORCID 0000-0001-5035-8201
Florian BuergerDepartment of Pediatrics, Boston Children's Hospital, Harvard Medical School, Boston, MA 02115, USA.
Padraic RomfhPendar Technologies, Cambridge, MA 02138, USA.ORCID 0000-0003-2502-9822
Ulrich KneserDepartment of Hand, Plastic and Reconstructive Surgery, Microsurgery, Burn Trauma Center, BG Trauma Center Ludwigshafen, University of Heidelberg, 67071 Ludwigshafen, Germany.ORCID 0000-0002-3402-2860
Brian D PolizzottiDepartment of Cardiology, Boston Children's Hospital, Harvard Medical School, Boston, MA 02115, USA.
Bohdan PomahacDivision of Plastic and Reconstructive Surgery, Yale University School of Medicine, New Haven, CT 06510, USA.
Boston Children's Hospital · USBrigham and Women's Hospital · USHeidelberg University · DEPendar Technologies (United States) · USUniversity of Calgary · CAYale University · US

Funding

Deutsche Forschungsgemeinschaft HA-8908/1-1
6 · The paper itself

Abstract

backgroundNormothermic ex situ perfusion of vascularized composite allografts (VCAs) necessitates high oxygen demand and, thus, increased metabolic activity, which, in turn, requires the use of blood-based perfusion solutions. However, blood-derived perfusates, in turn, constitute an antigenic load. To circumvent this immunogenic problem, we used a perfusate enriched with acellular dextrane oxygen microcarriers to perfuse rat hindlimbs.

methodsRat hindlimbs (n = 11) were perfused with either (non-), oxygenated dextrane-enriched Phoxilium, or Phoxilium enriched with dextrane oxygen microcarriers (MO

resultsFor all evaluated conditions, ischemia time until perfusion was comparable (22.91 ± 1.64 min;

conclusionThe use of oxygen microcarriers in ex situ perfusion of VCA with acellular perfusates under normothermic conditions for 12 h facilitates the maintenance of mitochondrial structure, as well as a subsequent recovery of mitochondrial redox status over time, while markers of muscle injury were lower compared to conventional oxygenated acellular perfusates.

Indexed as

ex situ perfusionex situ tissue preservationoxygen microcarriersRaman spectroscopyreal-time mitochondrial redox state quantificationvascularized composite allograft

Identifiers

PMID37892706
PMCPMC10607057
OpenAlexW4387702469

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.