Evidence map›Paper›PMID 37891687›Full record

ArticleBMC research notes2023

Expression of dengue virus and Zika virus NS2B-NS3pro constructs alter cellular fatty acids, but co-expression with a Zika virus virus-like particle is detrimental to virus-like particle expression.

Suwipa Ramphan, Nathamon Yimpring, Chontida Tangsongcharoen, Suthatta Sornprasert, Atitaya Hitakarun, Wannapa Sornjai, Sittiruk Roytrakul, Atikorn Panya, Duncan R Smith

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC research notes, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Suwipa Ramphan *Institute of Molecular Biosciences, Mahidol University, Salaya, 73170, Thailand.
Nathamon Yimpring *Institute of Molecular Biosciences, Mahidol University, Salaya, 73170, Thailand.
Chontida TangsongcharoenDepartment of Medical Technology, Faculty of Allied Health Sciences, Burapha University, Chonburi, 20130, Thailand.
Suthatta SornprasertInstitute of Molecular Biosciences, Mahidol University, Salaya, 73170, Thailand.
Atitaya HitakarunInstitute of Molecular Biosciences, Mahidol University, Salaya, 73170, Thailand.
Wannapa SornjaiInstitute of Molecular Biosciences, Mahidol University, Salaya, 73170, Thailand.
Sittiruk RoytrakulFunctional Proteomics Technology, National Center for Genetic Engineering and Biotechnology (BIOTECH), Thailand Science Park, Pathumthani, 12120, Thailand.
Atikorn PanyaFood Biotechnology Research Team, Functional Ingredients and Food Innovation Research Group, National Center for Genetic Engineering and Biotechnology (BIOTEC), Science Park, Pathumthani, 12120, Thailand.
Duncan R SmithInstitute of Molecular Biosciences, Mahidol University, Salaya, 73170, Thailand. duncan_r_smith@hotmail.com.
Mahidol University · THNational Center for Genetic Engineering and Biotechnology · THBurapha University · TH

Funding

Mahidol University BRF1-088/2565National Research Council of Thailand NRCT5-RGJ63012-139National Science and Technology Development Agency FDA-CO-2561-6820-THThailand Graduate Institute of Science and Technology TG-22-14-59-005DThailand Research Fund IRN5802PHDW03 under IRN58W0002
6 · The paper itself

Abstract

objectiveStudies have shown that Flavivirus infection remodels the host cell to favour viral replication. In particular, the host cell lipid profile is altered, and it has been proposed that this process alters membrane fluidity to allow wrapping of the outer structural proteins around the viral nucleocapsid. We investigated whether expression of the Zika virus (ZIKV) and dengue virus (DENV) protease induced alterations in the cellular lipid profile, and subsequently whether co-expression of these proteases with VLP constructs was able to improve VLP yield.

resultsOur results showed that both ZIKV and DENV proteases induced alterations in the lipid profile, but that both active and inactive proteases induced many of the same changes. Neither co-transfection of protease and VLP constructs nor bicistronic vectors allowing expression of both protease and VLP separated by a cell cleavable linker improved VLP yield, and indeed many of the constructs showed significantly reduced VLP production. Further work in developing improved VLP expression platforms is required.

Indexed as

Dengue VirusZika VirusZika Virus InfectionHumansLipidsPeptide HydrolasesViral Nonstructural ProteinsLipidsPeptide HydrolasesViral Nonstructural ProteinsDengue virusFatty acid methyl estersLipidNS3 proteaseVirus-like particlesZika virus

Identifiers

PMID37891687
PMCPMC10605870
OpenAlexW4387974074

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.