ArticleACS nano2023
High-Resolution Electron Diffraction of Hydrated Protein Crystals at Room Temperature.
Article in ACS nano, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- Advances of 3D microcrystals electron diffraction for transmembrane protein structure determination.Biophysical journal · 2026Review
- Sub-Ångström Three-Dimensional Electron Diffraction Reveals Crystal Structures and Phase Transformations in Liquids.Journal of the American Chemical Society · 2026Article
- Room-temperature structure determination of vacuum-sensitive organic compounds by formvar encapsulation and serial electron diffraction.Journal of applied crystallography · 2025Article
- Machine learning for the classification of serial electron diffraction patterns: synthetic data.Acta crystallographica. Section A, Foundations and advances · 2025Article
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Authors and funding
12 authors.
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Abstract
Structural characterization is crucial to understanding protein function. Compared with X-ray diffraction methods, electron crystallography can be performed on nanometer-sized crystals and can provide additional information from the resulting Coulomb potential map. Whereas electron crystallography has successfully resolved three-dimensional structures of vitrified protein crystals, its widespread use as a structural biology tool has been limited. One main reason is the fragility of such crystals. Protein crystals can be easily damaged by mechanical stress, change in temperature, or buffer conditions as well as by electron irradiation. This work demonstrates a methodology to preserve these nanocrystals in their natural environment at room temperature for electron diffraction experiments as an alternative to existing cryogenic techniques. Lysozyme crystals in their crystallization solution are hermetically sealed
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