ArticleScience advances2023
Stimulated Raman photothermal microscopy toward ultrasensitive chemical imaging.
Article in Science advances, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed.
- Longitudinal live-cell chemical imaging using coherent Raman scattering microscopy.The Analyst · 2026Review
- Submicromolar imaging of intrinsic chromophores by two-photon photothermal microscopy captures mitochondrial response to chemotherapy.Science advances · 2026Article
- Stimulated Raman photothermal microscopy: theory and implementation.Advances in optics and photonics · 2026Article
- The Next Frontier in Vibrational Imaging: Chemically Designed Raman Probes Pass the Spectral Barrier.JACS Au · 2026Review
- Rapid Antifungal Susceptibility Testing by Stimulated Raman Photothermal Imaging of DAnalytical chemistry · 2026Article
- Article
- Surface-enhanced stimulated Raman scattering and fluorescence probing of plasmonic nanoparticles in cellular environments: insights into their spatial distribution and aggregation.Nanoscale advances · 2026Article
- Far-field single-molecule vibrational spectroscopy and imaging.Chemical science · 2026Review
- Label-Free Molecular Characterization of Protein Aggregates in Differentiated Astrocytes.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Label-free molecular profiling of cancer using Raman spectroscopy: from fundamentals to clinical applications.Frontiers in oncology · 2026Review
- Recent advances in Raman probes for multiplexed bioimaging.Inflammation and regeneration · 2025Review
- Feeling the vibes: Vibrational spectroscopic imaging of biomolecular assemblies in their natural environment.Applied physics reviews · 2025Article
- Spontaneous or Stimulated? Investigating Raman's Detection Limits in Aqueous Environments.The journal of physical chemistry. B · 2025Article
- Single-Molecule Vibrational Thermometry.The journal of physical chemistry. B · 2025Article
- Self-Supervised Elimination of Non-Independent Noise in Hyperspectral Imaging.Newton ((New York, N.Y.) · 2025Article
- Self-stacked small molecules for ultrasensitive, substrate-free Raman imaging in vivo.Nature biotechnology · 2025Article
- Quantum theory of stimulated Raman scattering microscopy.Chemical physics reviews · 2025Review
- Raman imaging as a window into cellular complexity: a future perspective.Nature methods · 2025Article
- Advanced vibrational microscopes for life science.Nature methods · 2025Review
- A 20-year journey on the invention of vibrational photothermal microscopy.Nature methods · 2025Article
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Authors and funding
11 authors.
Funding
Abstract
Stimulated Raman scattering (SRS) microscopy has shown enormous potential in revealing molecular structures, dynamics, and couplings in complex systems. However, the sensitivity of SRS is fundamentally limited to the millimolar level due to shot noise and the small modulation depth. To overcome this barrier, we revisit SRS from the perspective of energy deposition. The SRS process pumps molecules to their vibrationally excited states. The subsequent relaxation heats up the surroundings and induces refractive index changes. By probing the refractive index changes with a laser beam, we introduce stimulated Raman photothermal (SRP) microscopy, where a >500-fold boost of modulation depth is achieved. The versatile applications of SRP microscopy on viral particles, cells, and tissues are demonstrated. SRP microscopy opens a way to perform vibrational spectroscopic imaging with ultrahigh sensitivity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.