Evidence map›Paper›PMID 37889390›Full record

ArticleFish physiology and biochemistry2023

Purification and characterization of amylases from three freshwater fish species providing new insight application as enzyme molecular markers for zymography.

Suthathip Phetlum, Chamaiporn Champasri

Abstract read
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In one paragraph

Article in Fish physiology and biochemistry, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.6field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 6 citations in OpenAlex.

  1. Electrophoretic Zymography of Protease Using Azocasein.Methods in molecular biology (Clifton, N.J.) · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Suthathip PhetlumDepartment of Biochemistry, Faculty of Science, Khon Kaen University, Khon Kaen, 40002, Thailand.
Chamaiporn ChampasriDepartment of Biochemistry, Faculty of Science, Khon Kaen University, Khon Kaen, 40002, Thailand. chamai@kku.ac.th.ORCID http://orcid.org/0000-0003-3516-0656
Khon Kaen University · TH

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Purification of amylases from digestive tracts of three freshwater fish species with Q-Sepharose Fast Flow and Sephacryl S-200 columns displayed two isoforms of amylases from Osteochilus hasselti (O1, O2) and three isoforms of those from both Hampala dispar (UB, H1, H2) and Puntioplites proctozystron (P1, P2, P3). The optimum pH values displayed at 7.0 and 8.0, while the optimum temperatures revealed at 40 and 50 °C. Almost isoenzyme activities were activated by NaCl and CaCl

Indexed as

alpha-AmylasesAmylasesAnimalsChromatography, LiquidEnzyme StabilityHydrogen-Ion ConcentrationIsoenzymesMolecular WeightTandem Mass SpectrometryTemperaturealpha-AmylasesAmylasesIsoenzymesAmylaseAmylase applicationFish enzymeIsoenzymeZymography

Identifiers

PMID37889390
OpenAlexW4387966076

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.