Evidence map›Paper›PMID 37888289›Full record

ArticleJournal of fungi (Basel, Switzerland)2023

Histone Abundance Quantification via Flow Cytometry of Htb2-GFP Allows Easy Monitoring of Cell Cycle Perturbations in Living Yeast Cells, Comparable to Standard DNA Staining.

Maria V Kulakova, Eslam S M O Ghazy, Fedor Ryabov, Yaroslav M Stanishevskiy, Michael O Agaphonov, Alexander I Alexandrov

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of fungi (Basel, Switzerland), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. ACS omega · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Maria V KulakovaFederal Research Center of Biotechnology of the RAS, Bach Institute of Biochemistry, Leninskiy Ave. 33, Moscow 119071, Russia.
Eslam S M O GhazyFederal Research Center of Biotechnology of the RAS, Bach Institute of Biochemistry, Leninskiy Ave. 33, Moscow 119071, Russia.
Fedor RyabovFederal Research Center of Biotechnology of the RAS, Bach Institute of Biochemistry, Leninskiy Ave. 33, Moscow 119071, Russia.ORCID 0000-0001-8728-9465
Yaroslav M StanishevskiyInstitute of Biochemical Technology and Nanotechnology, Peoples' Friendship University of Russia (RUDN), 6 Miklukho-Maklaya Street, Moscow 117198, Russia.
Michael O AgaphonovFederal Research Center of Biotechnology of the RAS, Bach Institute of Biochemistry, Leninskiy Ave. 33, Moscow 119071, Russia.ORCID 0000-0002-9270-9476
Alexander I AlexandrovFederal Research Center of Biotechnology of the RAS, Bach Institute of Biochemistry, Leninskiy Ave. 33, Moscow 119071, Russia.ORCID 0000-0001-7250-4740

Funding

Russian Science Foundation 22-24-00756
6 · The paper itself

Abstract

Assaying changes in the amount of DNA in single cells is a well-established method for studying the effects of various perturbations on the cell cycle. A drawback of this method is the need for a fixation procedure that does not allow for in vivo study nor simultaneous monitoring of additional parameters such as fluorescence of tagged proteins or genetically encoded indicators. In this work, we report on a method of Histone Abundance Quantification (HAQ) of live yeast harboring a GFP-tagged histone, Htb2. We show that it provides data highly congruent with DNA levels, both in

Indexed as

cell cycleflow cytometryhistoneOgataeaSaccharomycesyeast

Identifiers

PMID37888289
PMCPMC10608138

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.