Evidence map›Paper›PMID 37887103›Full record

ArticleBiosensors2023

utPCR: A Strategy for the Highly Specific and Absolutely Quantitative Detection of Single Molecules within Only Minutes.

Rui Wang, Ying Liu, Shuaiwei Chen, Linlin Bai, Kaiming Guo, Yanan Pang, Feng Qian, Yongfang Li, Li Ding, Yongming Wang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biosensors, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Making Molecular Diagnostics Faster.International journal of laboratory hematology · 2026
    Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Rui WangState Key Laboratory of Genetic Engineering, School of Life Sciences, Zhongshan Hospital, Human Phenome Institute, Pudong Hospital, Fudan University, Shanghai 200438, China.ORCID 0000-0003-2945-2046
Ying LiuState Key Laboratory of Genetic Engineering, School of Life Sciences, Zhongshan Hospital, Human Phenome Institute, Pudong Hospital, Fudan University, Shanghai 200438, China.ORCID 0009-0009-5019-6383
Shuaiwei ChenState Key Laboratory of Genetic Engineering, School of Life Sciences, Zhongshan Hospital, Human Phenome Institute, Pudong Hospital, Fudan University, Shanghai 200438, China.
Linlin BaiState Key Laboratory of Genetic Engineering, School of Life Sciences, Zhongshan Hospital, Human Phenome Institute, Pudong Hospital, Fudan University, Shanghai 200438, China.
Kaiming GuoState Key Laboratory of Genetic Engineering, School of Life Sciences, Zhongshan Hospital, Human Phenome Institute, Pudong Hospital, Fudan University, Shanghai 200438, China.
Yanan PangChanghai Hospital, Second Military Medical University, Shanghai 200433, China.
Feng QianState Key Laboratory of Genetic Engineering, School of Life Sciences, Zhongshan Hospital, Human Phenome Institute, Pudong Hospital, Fudan University, Shanghai 200438, China.
Yongfang LiSchool of Food Science and Engineering, Foshan University, Foshan 528231, China.
Li DingChanghai Hospital, Second Military Medical University, Shanghai 200433, China.
Yongming WangState Key Laboratory of Genetic Engineering, School of Life Sciences, Zhongshan Hospital, Human Phenome Institute, Pudong Hospital, Fudan University, Shanghai 200438, China.ORCID 0000-0001-8269-5296

Funding

the National Natural Science Foundation of China 32001786，82103268, 82070258the Science and Technology Commission of Shanghai Municipality 23QA1404300，22Y11909200，21YF1459100，20490780100
6 · The paper itself

Abstract

Bloodstream infection is a major health problem worldwide, with extremely high mortality. Detecting infection in the early stage is challenging due to the extremely low concentration of bacteria in the blood. Digital PCR provides unparalleled sensitivity and can achieve absolute quantification, but it is time-consuming. Moreover, the presence of unavoidable background signals in negative controls poses a significant challenge for single-molecule detection. Here, we propose a novel strategy called "Ultrafast flexible thin tube-based droplet digital PCR (utPCR)" that can shorten the digital PCR process from 2 h to only 5 min, with primer annealing/extension time reduced from minutes to only 5 s. Importantly, the ultrafast PCR eliminates nonspecific amplification and thus enables single-molecule detection. The utPCR enabled the sensitive detection and digital quantification of

Indexed as

Escherichia coli K12Escherichia coli O157SepsisHumansLimit of DetectionPolymerase Chain Reactionabsolute quantificationbloodstream infectiondroplet digital PCR (ddPCR)molecular diagnosissingle-molecule detectionultrafast PCR

Identifiers

PMID37887103
PMCPMC10605045

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.